SeedGenes Profile: EMB 2284

Gene Information Gene Symbol EMB 2284 Chromosome Locus At1g08260   Alleles in Database 7   See List OR Show All Identity Confidence Confirmed Alias Symbol TIL 1 (P. Jenik) Sequence Identity EMB 529 (M. Devic) Sequence Identity TIL 1 (P. Jenik) Complemetation Test EMB 142 (D. Meinke) Complemetation Test Gene Class Embryo Defective Predicted Function Subunit of DNA Polymerase Epsilon Function Details DNA replication and perhaps DNA repair and cell cycle control Gene Evidence Border Recovery D. Meinke (1 allele) Double Border Recovery Meinke / Syngenta (3 alleles) Border Recovery Devic / Salk (1 allele) Border Recovery Devic / Versailles (1 allele) Sequenced Mutant Allele P. Jenik (1 allele) Database Release March, 2002 Predicted Sequences Gene: 15949 nucleotides mRNA: 6816 nucleotides Protein:   2271 amino acids cDNA Status EST Identified Top BLAST Hits Arabidopsis: AT2G27120.1 (79%) Plant ESTs: Rice (Oryza sativa) (57%) Model Genomes:   Homo sapiens (44%) Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 261811_at

Mutant Information Allele Symbol emb 2284-1 emb 2284-2 emb 2284-3 emb 529 emb 2284-5 til 1-4 emb 142 Mutant Class Embryo Defective Embryo Defective Embryo Defective Embryo Defective Embryo Defective Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Syngenta Meinke / Syngenta Devic / Versailles Devic / Salk Jenik / Joy Meinke / DuPont Mutant Line Number 76172 63003 67320   SALK_057024   2977 Ecotype Columbia Columbia Columbia Wassilewskija Columbia   Wassilewskija Genetic Background qrt qrt qrt         ABRC Stock CS16046 CS16142 CS16143         Mutagen Treatment T-DNA T-DNA T-DNA T-DNA T-DNA EMS T-DNA Insertion Mutant Yes   Details Yes   Details Yes   Details Yes   Details Yes   Details No Yes   Details Flanking Sequence Details  (Help) Details Details         Location of Mutation Intron 44 Intron 47 to Exon 48 Intron 7 Intron 25 5' UTR Exon 12 Exon 3 Terminal Phenotype Globular  Details Globular  Details Globular  Details Globular Globular Cotyledon Globular Nomarski Phenotype   Images   Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Complementation Test; Sequence Identity Complementation Test; Sequence Identity Complementation Test; Sequence Identity Complementation Test; Sequence Identity Complementation Test; Sequence Identity Database Release March, 2002 September, 2002 September, 2002 October, 2007 October, 2007 October, 2007 December, 2010 Seed Color Pale Yellow Pale Yellow Pale Yellow         Embryo Color Pale Yellow Pale Yellow Pale Yellow         Percent Mutant Seeds 25.9 % 26.4 % 24.0 %       27.3 % Chi - Square 0.4 1.2 0.6         Seeds Counted 912 1163 1108         Percent Top Half 55.1 % 53.4 % 51.1 %       47.3 % Chi - Square 2.4 1.4 0.1         Average Seed Length 480 ± 40 µm 510 ± 50 µm 490 ± 50 µm         Average Embryo Length 60 ± 20 µm 80 ± 40 µm 70 ± 20 µm