SeedGenes Profile: EMB 2284

Gene Information Gene Symbol EMB 2284 Chromosome Locus At1g08260   Alleles in Database 7   See List OR Show All Identity Confidence Confirmed Alias Symbol TIL 1 (P. Jenik) Sequence Identity EMB 529 (M. Devic) Sequence Identity TIL 1 (P. Jenik) Complemetation Test EMB 142 (D. Meinke) Complemetation Test Gene Class Embryo Defective Predicted Function Subunit of DNA Polymerase Epsilon Function Details DNA replication and perhaps DNA repair and cell cycle control Gene Evidence Border Recovery D. Meinke (1 allele) Double Border Recovery Meinke / Syngenta (3 alleles) Border Recovery Devic / Salk (1 allele) Border Recovery Devic / Versailles (1 allele) Sequenced Mutant Allele P. Jenik (1 allele) Database Release March, 2002 Predicted Sequences Gene: 15949 nucleotides mRNA: 6816 nucleotides Protein:   2271 amino acids cDNA Status EST Identified Top BLAST Hits Arabidopsis: AT2G27120.1 (79%) Plant ESTs: Rice (Oryza sativa) (57%) Model Genomes:   Homo sapiens (44%) Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 261811_at

Mutant Information Allele Symbol emb 2284-2 Mutant Class Embryo Defective Source of Mutant Meinke / Syngenta Mutant Line Number 63003 Ecotype Columbia Genetic Background qrt ABRC Stock CS16142 Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence Details  (Help) Location of Mutation Intron 47 to Exon 48 Terminal Phenotype Globular  Details Nomarski Phenotype   Images Allelism Evidence Complementation Test; Sequence Identity Database Release September, 2002 Seed Color Pale Yellow Embryo Color Pale Yellow Percent Mutant Seeds 26.4 % Chi - Square 1.2 Seeds Counted 1163 Percent Top Half 53.4 % Chi - Square 1.4 Average Seed Length 510 ± 50 µm Average Embryo Length 80 ± 40 µm