OSU Syngenta vbi

SeedGenes Profile: EMB 2284

Gene Information
Gene Symbol EMB 2284
Chromosome Locus At1g08260   tair
Alleles in Database 7   See List OR Show All
Identity Confidence Confirmed
Alias Symbol TIL 1 (P. Jenik) Sequence Identity
EMB 529 (M. Devic) Sequence Identity
TIL 1 (P. Jenik) Complemetation Test
EMB 142 (D. Meinke) Complemetation Test
Gene Class Embryo Defective
Predicted Function Subunit of DNA Polymerase Epsilon
Function Details DNA replication and perhaps DNA repair and cell cycle control
Gene Evidence
Border Recovery
D. Meinke (1 allele)
Double Border Recovery
Meinke / Syngenta (3 alleles)
Border Recovery
Devic / Salk (1 allele)
Border Recovery
Devic / Versailles (1 allele)
Sequenced Mutant Allele
P. Jenik (1 allele)
Database Release March, 2002
Predicted Sequences
Gene:15949 nucleotides
mRNA: 6816 nucleotides
Protein:  2271 amino acids
cDNA Status EST Identified
Top BLAST Hits
Arabidopsis: AT2G27120.1 (79%)
Plant ESTs: Rice (Oryza sativa) (57%)
Model Genomes:  Homo sapiens (44%)
Subcellular Localization TargetP Not Targeted to Cell Compartment
mRNA Expression Data NASCArray Spot History for 261811_at
Mutant Information
Allele Symbol emb 2284-2
Mutant Class Embryo Defective
Source of Mutant Meinke / Syngenta
Mutant Line Number 63003
Ecotype Columbia
Genetic Background qrt
ABRC Stock CS16142
Mutagen Treatment T-DNA
Insertion Mutant Yes   Details
Flanking Sequence Details  (Help)
Location of Mutation Intron 47 to Exon 48
Terminal Phenotype Globular  Details
Nomarski Phenotype   Images
Allelism Evidence Complementation Test; Sequence Identity
Database Release September, 2002
Seed Color Pale Yellow
Embryo Color Pale Yellow
Percent Mutant Seeds 26.4 %
Chi - Square 1.2
Seeds Counted 1163
Percent Top Half 53.4 %
Chi - Square 1.4
Average Seed Length 510 ± 50 µm
Average Embryo Length 80 ± 40 µm