SeedGenes Profile: EMB 2289

Gene Information Gene Symbol EMB 2289 Chromosome Locus At2g31530   Alleles in Database 3   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Chloroplast Protein Translocase (SECY2) Function Details Translocation of nuclear-encoded proteins; cotranslational membrane protein translocation and insertion in chloroplasts Gene Evidence Single Border Recovery Meinke / Syngenta (1 allele) Double Border Recovery Meinke / Syngenta (2 alleles) Database Release March, 2002 Predicted Sequences Gene: 3058 nucleotides mRNA: 1828 nucleotides Protein:   556 amino acids cDNA Status FLcDNA Available: BX820289 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Vitis vinifera (71%) Model Genomes:   Synechocystis (30%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 263425_at

Mutant Information Allele Symbol emb 2289-2 Mutant Class Embryo Defective Source of Mutant Meinke / Syngenta Mutant Line Number 78584 Ecotype Columbia Genetic Background qrt ABRC Stock CS16048 Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence Details  (Help) Location of Mutation Intron 9 Terminal Phenotype Globular  Details Nomarski Phenotype   Images Allelism Evidence Complementation Test; Sequence Identity Database Release March, 2002 Seed Color White Embryo Color White Percent Mutant Seeds 23.0 % Chi - Square 2.6 Seeds Counted 1163 Percent Top Half 55.0 % Chi - Square 2.7 Average Seed Length 460 ± 20 µm Average Embryo Length 50 ± 10 µm