OSU Syngenta vbi

SeedGenes Profile: EMB 2289

Gene Information
Gene Symbol EMB 2289
Chromosome Locus At2g31530   tair
Alleles in Database 3   See List OR Show All
Identity Confidence Confirmed
Alias Symbol  
Gene Class Embryo Defective
Predicted Function Chloroplast Protein Translocase (SECY2)
Function Details Translocation of nuclear-encoded proteins; cotranslational membrane protein translocation and insertion in chloroplasts
Gene Evidence
Single Border Recovery
Meinke / Syngenta (1 allele)
Double Border Recovery
Meinke / Syngenta (2 alleles)
Database Release March, 2002
Predicted Sequences
Gene:3058 nucleotides
mRNA: 1828 nucleotides
Protein:  556 amino acids
cDNA Status FLcDNA Available: BX820289
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Vitis vinifera (71%)
Model Genomes:  Synechocystis (30%)
Subcellular Localization TargetP Targeted to Chloroplasts
SUBA
mRNA Expression Data NASCArray Spot History for 263425_at
    
Mutant Information
Allele Symbol emb 2289-1emb 2289-2emb 2289-3
Mutant Class Embryo DefectiveEmbryo DefectiveEmbryo Defective
Source of Mutant Meinke / SyngentaMeinke / SyngentaMeinke / Syngenta
Mutant Line Number 767227858424018
Ecotype ColumbiaColumbiaColumbia
Genetic Background qrtqrt 
ABRC Stock CS16047CS16048CS16049
Mutagen Treatment T-DNAT-DNAT-DNA
Insertion Mutant Yes   DetailsYes   DetailsYes   Details
Flanking Sequence Details  (Help)DetailsDetails
Location of Mutation Intron 2Intron 9Intron 1
Terminal Phenotype Globular  DetailsGlobular  DetailsGlobular  Details
Nomarski Phenotype   Images  Images  Images
Allelism Evidence Reference AlleleComplementation Test; Sequence IdentityComplementation Test; Sequence Identity
Database Release March, 2002March, 2002March, 2002
Seed Color WhiteWhiteWhite
Embryo Color WhiteWhiteWhite
Percent Mutant Seeds 22.2 %23.0 %24.0 %
Chi - Square 10.72.60.6
Seeds Counted 96411631059
Percent Top Half 47.3 %55.0 %51.6 %
Chi - Square 0.62.70.3
Average Seed Length 440 ± 30 µm460 ± 20 µm450 ± 20 µm
Average Embryo Length 50 ± 10 µm50 ± 10 µm50 ± 10 µm