SeedGenes Profile: EMB 2289

Gene Information Gene Symbol EMB 2289 Chromosome Locus At2g31530   Alleles in Database 3   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Chloroplast Protein Translocase (SECY2) Function Details Translocation of nuclear-encoded proteins; cotranslational membrane protein translocation and insertion in chloroplasts Gene Evidence Single Border Recovery Meinke / Syngenta (1 allele) Double Border Recovery Meinke / Syngenta (2 alleles) Database Release March, 2002 Predicted Sequences Gene: 3058 nucleotides mRNA: 1828 nucleotides Protein:   556 amino acids cDNA Status FLcDNA Available: BX820289 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Vitis vinifera (71%) Model Genomes:   Synechocystis (30%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 263425_at

Mutant Information Allele Symbol emb 2289-1 emb 2289-2 emb 2289-3 Mutant Class Embryo Defective Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Syngenta Meinke / Syngenta Mutant Line Number 76722 78584 24018 Ecotype Columbia Columbia Columbia Genetic Background qrt qrt   ABRC Stock CS16047 CS16048 CS16049 Mutagen Treatment T-DNA T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Yes   Details Flanking Sequence Details  (Help) Details Details Location of Mutation Intron 2 Intron 9 Intron 1 Terminal Phenotype Globular  Details Globular  Details Globular  Details Nomarski Phenotype   Images   Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Complementation Test; Sequence Identity Database Release March, 2002 March, 2002 March, 2002 Seed Color White White White Embryo Color White White White Percent Mutant Seeds 22.2 % 23.0 % 24.0 % Chi - Square 10.7 2.6 0.6 Seeds Counted 964 1163 1059 Percent Top Half 47.3 % 55.0 % 51.6 % Chi - Square 0.6 2.7 0.3 Average Seed Length 440 ± 30 µm 460 ± 20 µm 450 ± 20 µm Average Embryo Length 50 ± 10 µm 50 ± 10 µm 50 ± 10 µm