OSU Syngenta vbi

SeedGenes Profile: EMB 2289

Gene Information
Gene Symbol EMB 2289
Chromosome Locus At2g31530   tair
Alleles in Database 3   See List OR Show All
Identity Confidence Confirmed
Alias Symbol  
Gene Class Embryo Defective
Predicted Function Chloroplast Protein Translocase (SECY2)
Function Details Translocation of nuclear-encoded proteins; cotranslational membrane protein translocation and insertion in chloroplasts
Gene Evidence
Single Border Recovery
Meinke / Syngenta (1 allele)
Double Border Recovery
Meinke / Syngenta (2 alleles)
Database Release March, 2002
Predicted Sequences
Gene:3058 nucleotides
mRNA: 1828 nucleotides
Protein:  556 amino acids
cDNA Status FLcDNA Available: BX820289
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Vitis vinifera (71%)
Model Genomes:  Synechocystis (30%)
Subcellular Localization TargetP Targeted to Chloroplasts
mRNA Expression Data NASCArray Spot History for 263425_at
Mutant Information
Allele Symbol emb 2289-3
Mutant Class Embryo Defective
Source of Mutant Meinke / Syngenta
Mutant Line Number 24018
Ecotype Columbia
Genetic Background  
ABRC Stock CS16049
Mutagen Treatment T-DNA
Insertion Mutant Yes   Details
Flanking Sequence Details  (Help)
Location of Mutation Intron 1
Terminal Phenotype Globular  Details
Nomarski Phenotype   Images
Allelism Evidence Complementation Test; Sequence Identity
Database Release March, 2002
Seed Color White
Embryo Color White
Percent Mutant Seeds 24.0 %
Chi - Square 0.6
Seeds Counted 1059
Percent Top Half 51.6 %
Chi - Square 0.3
Average Seed Length 450 ± 20 µm
Average Embryo Length 50 ± 10 µm