SeedGenes Profile: EMB 2369

Gene Information Gene Symbol EMB 2369 Chromosome Locus At4g04350   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Leucyl tRNA Synthetase Function Details Translation in chloroplast Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Single Border Recovery Meinke / Genoplante (1 allele) Database Release March, 2004 Predicted Sequences Gene: 5040 nucleotides mRNA: 3060 nucleotides Protein:   973 amino acids cDNA Status FLcDNA Available: AY079159 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Vitis vinifera (79%) Model Genomes:   Synechocystis (45%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 255328_at

Mutant Information Allele Symbol emb 2369-1 Mutant Class Embryo Defective Source of Mutant Meinke / Syngenta Mutant Line Number 81281 Ecotype Columbia Genetic Background qrt ABRC Stock CS24052 Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence Details  (Help) Location of Mutation Intron 9 to Exon 10 Terminal Phenotype Globular  Details Nomarski Phenotype   Images Allelism Evidence Reference Allele Database Release March, 2004 Seed Color White Embryo Color White Percent Mutant Seeds 24.8 % Chi - Square <0.1 Seeds Counted 1064 Percent Top Half 45.8 % Chi - Square 1.8 Average Seed Length 530 ± 70 µm Average Embryo Length 80 ± 30 µm