OSU Syngenta vbi

SeedGenes Profile: EMB 2448

Gene Information
Gene Symbol EMB 2448
Chromosome Locus At4g11150   tair
Alleles in Database 3   See List OR Show All
Identity Confidence Confirmed
Alias Symbol VHAE 1 (K. Schumacher) Sequence Identity
TUF (U. Mayer) Sequence Identity
Gene Class Embryo Defective
Predicted Function Vacuolar H+ ATPase (VHA-E1)
Function Details Vacuolar proton pump component
Gene Evidence
Double Border Recovery
Meinke / Syngenta (1 allele)
Sequenced Mutant Allele
U. Mayer (1 allele)
Molecular Complementation
U. Mayer (1 allele)
Border Recovery
Mayer / Salk (1 allele)
Database Release March, 2003
Predicted Sequences
Gene:1998 nucleotides
mRNA: 1085 nucleotides
Protein:  230 amino acids
cDNA Status FLcDNA Available: AY065119
Top BLAST Hits
Arabidopsis: AT1G64200.1 (80%)
Plant ESTs: Brassica napus (94%)
Model Genomes:  Drosophila melanogaster (44%)
Subcellular Localization TargetP Not Targeted to Cell Compartment
mRNA Expression Data NASCArray Spot History for 254903_at
Mutant Information
Allele Symbol emb 2448
Mutant Class Embryo Defective
Source of Mutant Meinke / Syngenta
Mutant Line Number 88248
Ecotype Columbia
Genetic Background  
ABRC Stock CS16180
Mutagen Treatment T-DNA
Insertion Mutant Yes   Details
Flanking Sequence Details  (Help)
Location of Mutation Intron 3 to Intron 4
Terminal Phenotype Preglobular  Details
Nomarski Phenotype   Images
Allelism Evidence Reference Allele
Database Release March, 2003
Seed Color White to Pale Yellow
Embryo Color White to Pale Yellow
Percent Mutant Seeds 23.5 %
Chi - Square 1.2
Seeds Counted 966
Percent Top Half 51.1 %
Chi - Square 0.1
Average Seed Length 470 ± 40 µm
Average Embryo Length 15 ± 0 µm