SeedGenes Profile: EMB 2448

Gene Information Gene Symbol EMB 2448 Chromosome Locus At4g11150   Alleles in Database 3   See List OR Show All Identity Confidence Confirmed Alias Symbol VHAE 1 (K. Schumacher) Sequence Identity TUF (U. Mayer) Sequence Identity Gene Class Embryo Defective Predicted Function Vacuolar H+ ATPase (VHA-E1) Function Details Vacuolar proton pump component Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Sequenced Mutant Allele U. Mayer (1 allele) Molecular Complementation U. Mayer (1 allele) Border Recovery Mayer / Salk (1 allele) Database Release March, 2003 Predicted Sequences Gene: 1998 nucleotides mRNA: 1085 nucleotides Protein:   230 amino acids cDNA Status FLcDNA Available: AY065119 Top BLAST Hits Arabidopsis: AT1G64200.1 (80%) Plant ESTs: Brassica napus (94%) Model Genomes:   Drosophila melanogaster (44%) Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 254903_at

Mutant Information Allele Symbol emb 2448 Mutant Class Embryo Defective Source of Mutant Meinke / Syngenta Mutant Line Number 88248 Ecotype Columbia Genetic Background   ABRC Stock CS16180 Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence Details  (Help) Location of Mutation Intron 3 to Intron 4 Terminal Phenotype Preglobular  Details Nomarski Phenotype   Images Allelism Evidence Reference Allele Database Release March, 2003 Seed Color White to Pale Yellow Embryo Color White to Pale Yellow Percent Mutant Seeds 23.5 % Chi - Square 1.2 Seeds Counted 966 Percent Top Half 51.1 % Chi - Square 0.1 Average Seed Length 470 ± 40 µm Average Embryo Length 15 ± 0 µm