SeedGenes Profile: EMB 2454

Gene Information Gene Symbol EMB 2454 Chromosome Locus At3g18290   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Zinc Finger Protein Function Details Putative role in transcriptional regulation Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Single Border Recovery Meinke / Syngenta (1 allele) Database Release March, 2002 Predicted Sequences Gene: 6516 nucleotides mRNA: 4414 nucleotides Protein:   1254 amino acids cDNA Status FLcDNA Available: AY094471 Top BLAST Hits Arabidopsis: AT1G74770.1 (56%) Plant ESTs: Lotus japonicus (67%) Model Genomes:   Neurospora crassa (40%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 257062_at

Mutant Information Allele Symbol emb 2454-1 Mutant Class Embryo Defective Source of Mutant Meinke / Syngenta Mutant Line Number 60578.1 Ecotype Columbia Genetic Background   ABRC Stock CS16055 Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence Details  (Help) Location of Mutation Intron 5 Terminal Phenotype Preglobular  Details Nomarski Phenotype Globular  Images Allelism Evidence Reference Allele Database Release March, 2002 Seed Color White Embryo Color White Percent Mutant Seeds 28.1 % Chi - Square 5.6 Seeds Counted 1085 Percent Top Half 48.2 % Chi - Square 0.4 Average Seed Length 390 ± 60 µm Average Embryo Length 15 ± 0 µm