SeedGenes Profile: EMB 2454

Gene Information Gene Symbol EMB 2454 Chromosome Locus At3g18290   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Zinc Finger Protein Function Details Putative role in transcriptional regulation Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Single Border Recovery Meinke / Syngenta (1 allele) Database Release March, 2002 Predicted Sequences Gene: 6516 nucleotides mRNA: 4414 nucleotides Protein:   1254 amino acids cDNA Status FLcDNA Available: AY094471 Top BLAST Hits Arabidopsis: AT1G74770.1 (56%) Plant ESTs: Lotus japonicus (67%) Model Genomes:   Neurospora crassa (40%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 257062_at

Mutant Information Allele Symbol emb 2454-1 emb 2454-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Syngenta Mutant Line Number 60578.1 71954 Ecotype Columbia Columbia Genetic Background   qrt ABRC Stock CS16055 CS16056 Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence Details  (Help) Details Location of Mutation Intron 5 5' UTR Terminal Phenotype Preglobular  Details Transition  Details Nomarski Phenotype Globular  Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release March, 2002 March, 2002 Seed Color White White Embryo Color White White Percent Mutant Seeds 28.1 % 19.5 % Chi - Square 5.6 16.4 Seeds Counted 1085 1024 Percent Top Half 48.2 % 45.5 % Chi - Square 0.4 1.6 Average Seed Length 390 ± 60 µm 440 ± 30 µm Average Embryo Length 15 ± 0 µm 50 ± 50 µm