SeedGenes Profile: EMB 2730

Gene Information Gene Symbol EMB 2730 Chromosome Locus At5g02250   Alleles in Database 5   See List OR Show All Identity Confidence Confirmed Alias Symbol RNR 1 (D. Stern) Sequence Identity RNR 1 (D. Stern) Complemetation Test Gene Class Embryo Defective Predicted Function Ribonuclease II Family Protein Function Details 3' to 5' exoribonuclease; Maturation of chloroplast rRNAs Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Border Recovery Meinke / Salk (1 allele) Border Recovery Stern / Salk (2 alleles) Border Recovery Meinke; Stern / Salk (1 allele) Database Release September, 2002 Predicted Sequences Gene: 4745 nucleotides mRNA: 2911 nucleotides Protein:   803 amino acids cDNA Status FLcDNA Available: AK176383 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Rice (Oryza sativa) (58%) Model Genomes:   Synechocystis (37%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 251040_at

Mutant Information Allele Symbol emb 2730-1 Mutant Class Embryo Defective Source of Mutant Meinke / Syngenta Mutant Line Number 11129 Ecotype Columbia Genetic Background   ABRC Stock CS16126 Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence Details  (Help) Location of Mutation Exon 14 to 3' UTR Terminal Phenotype Cotyledon  Details Nomarski Phenotype   Images Allelism Evidence Reference Allele Database Release September, 2002 Seed Color White to Pale Yellow Embryo Color White to Pale Yellow Percent Mutant Seeds 24.0 % Chi - Square 0.6 Seeds Counted 1079 Percent Top Half 53.6 % Chi - Square 1.4 Average Seed Length 500 ± 40 µm Average Embryo Length 380 ± 60 µm