OSU Syngenta vbi

SeedGenes Profile: EMB 2730

Gene Information
Gene Symbol EMB 2730
Chromosome Locus At5g02250   tair
Alleles in Database 5   See List OR Show All
Identity Confidence Confirmed
Alias Symbol RNR 1 (D. Stern) Sequence Identity
RNR 1 (D. Stern) Complemetation Test
Gene Class Embryo Defective
Predicted Function Ribonuclease II Family Protein
Function Details 3' to 5' exoribonuclease; Maturation of chloroplast rRNAs
Gene Evidence
Double Border Recovery
Meinke / Syngenta (1 allele)
Border Recovery
Meinke / Salk (1 allele)
Border Recovery
Stern / Salk (2 alleles)
Border Recovery
Meinke; Stern / Salk (1 allele)
Database Release September, 2002
Predicted Sequences
Gene:4745 nucleotides
mRNA: 2911 nucleotides
Protein:  803 amino acids
cDNA Status FLcDNA Available: AK176383
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Rice (Oryza sativa) (58%)
Model Genomes:  Synechocystis (37%)
Subcellular Localization TargetP Targeted to Chloroplasts
mRNA Expression Data NASCArray Spot History for 251040_at
Mutant Information
Allele Symbol emb 2730-1
Mutant Class Embryo Defective
Source of Mutant Meinke / Syngenta
Mutant Line Number 11129
Ecotype Columbia
Genetic Background  
ABRC Stock CS16126
Mutagen Treatment T-DNA
Insertion Mutant Yes   Details
Flanking Sequence Details  (Help)
Location of Mutation Exon 14 to 3' UTR
Terminal Phenotype Cotyledon  Details
Nomarski Phenotype   Images
Allelism Evidence Reference Allele
Database Release September, 2002
Seed Color White to Pale Yellow
Embryo Color White to Pale Yellow
Percent Mutant Seeds 24.0 %
Chi - Square 0.6
Seeds Counted 1079
Percent Top Half 53.6 %
Chi - Square 1.4
Average Seed Length 500 ± 40 µm
Average Embryo Length 380 ± 60 µm