SeedGenes Profile: EMB 2737

Gene Information Gene Symbol EMB 2737 Chromosome Locus At5g53860   Alleles in Database 3   See List OR Show All Identity Confidence Confirmed Alias Symbol EMB 64 (D. Meinke) Complemetation Test Gene Class Embryo Defective Predicted Function Unknown Function Details Putative auxin-regulated protein Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Single Border Recovery Meinke / Salk (1 allele) Database Release March, 2003 Predicted Sequences Gene: 3575 nucleotides mRNA: 1620 nucleotides Protein:   422 amino acids cDNA Status FLcDNA Available: AY050429 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Cotton (Gossypium) (79%) Model Genomes:   None Subcellular Localization TargetP Targeted to Mitochondria SUBA mRNA Expression Data NASCArray Spot History for 248235_at

Mutant Information Allele Symbol emb 2737-1 emb 2737-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Salk Mutant Line Number 116042 SALK_083393 Ecotype Columbia Columbia Genetic Background qrt   ABRC Stock CS16187 CS24089 Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence Details  (Help)   Location of Mutation Intron 9 Intron 3 Terminal Phenotype Cotyledon  Details Cotyledon  Details Nomarski Phenotype   Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release March, 2003 March, 2004 Seed Color White White Embryo Color White White Percent Mutant Seeds 26.6 % 24.7 % Chi - Square 1.6 0.1 Seeds Counted 1182 1006 Percent Top Half 52.5 % 52.0 % Chi - Square 0.8 0.4 Average Seed Length 550 ± 40 µm 530 ± 60 µm Average Embryo Length 440 ± 60 µm 150 ± 80 µm