SeedGenes Profile: EMB 2738

Gene Information Gene Symbol EMB 2738 Chromosome Locus At3g12080   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Uncertain Function Details Putative ribosome biogenesis GTPase (EngA) Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Single Border Recovery Meinke / Salk (1 allele) Database Release March, 2003 Predicted Sequences Gene: 4347 nucleotides mRNA: 2209 nucleotides Protein:   663 amino acids cDNA Status FLcDNA Available: AY080655 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Spruce (69%) Model Genomes:   Synechocystis (46%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 256274_at

Mutant Information Allele Symbol emb 2738-1 emb 2738-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Salk Mutant Line Number 121994 SALK_104755 Ecotype Columbia Columbia Genetic Background     ABRC Stock CS16188 CS24090 Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence Details  (Help)   Location of Mutation Upstream Intron 6 Terminal Phenotype Globular  Details Globular  Details Nomarski Phenotype   Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release March, 2003 March, 2004 Seed Color White White Embryo Color White White Percent Mutant Seeds 24.7 % 25.2 % Chi - Square <0.1 <0.1 Seeds Counted 1062 1121 Percent Top Half 48.8 % 49.1 % Chi - Square 0.1 <0.1 Average Seed Length 520 ± 30 µm 500 ± 60 µm Average Embryo Length 100 ± 30 µm 70 ± 30 µm