OSU Syngenta vbi

SeedGenes Profile: EMB 2746

Gene Information
Gene Symbol EMB 2746
Chromosome Locus At5g63420   tair
Alleles in Database 2   See List OR Show All
Identity Confidence Confirmed
Alias Symbol  
Gene Class Embryo Defective
Predicted Function Metallo-Beta-Lactamase Family Protein
Function Details Putative chloroplast RNA endounclease (Rnase J)
Gene Evidence
Double Border Recovery
Meinke / Syngenta (1 allele)
Single Border Recovery
Meinke / Salk (1 allele)
Database Release March, 2003
Predicted Sequences
Gene:5583 nucleotides
mRNA: 3026 nucleotides
Protein:  911 amino acids
cDNA Status FLcDNA Available: AK221272
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Vitis vinifera (70%)
Model Genomes:  Synechocystis (33%)
Subcellular Localization TargetP Targeted to Chloroplasts
SUBA
mRNA Expression Data NASCArray Spot History for 247385_at
    
Mutant Information
Allele Symbol emb 2746-1emb 2746-2
Mutant Class Embryo DefectiveEmbryo Defective
Source of Mutant Meinke / SyngentaMeinke / Salk
Mutant Line Number 128084SALK_110582
Ecotype ColumbiaColumbia
Genetic Background   
ABRC Stock CS16191CS24091
Mutagen Treatment T-DNAT-DNA
Insertion Mutant Yes   DetailsYes   Details
Flanking Sequence Details  (Help) 
Location of Mutation Exon 9Intron 7
Terminal Phenotype Globular  DetailsGlobular  Details
Nomarski Phenotype   Images  Images
Allelism Evidence Reference AlleleComplementation Test; Sequence Identity
Database Release March, 2003March, 2004
Seed Color WhiteWhite
Embryo Color WhiteWhite
Percent Mutant Seeds 25.1 %24.0 %
Chi - Square <0.10.5
Seeds Counted 11741124
Percent Top Half 50.2 %49.6 %
Chi - Square <0.1<0.1
Average Seed Length 500 ± 40 µm500 ± 60 µm
Average Embryo Length 80 ± 20 µm70 ± 30 µm