SeedGenes Profile: EMB 2750

Gene Information Gene Symbol EMB 2750 Chromosome Locus At3g06430   Alleles in Database 4   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function PPR Protein Function Details Processing of organellar mRNAs Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Border Recovery Myouga / Riken (3 alleles) Database Release September, 2003 Predicted Sequences Gene: 1891 nucleotides mRNA: 1769 nucleotides Protein:   486 amino acids cDNA Status FLcDNA Available: AY099616 Top BLAST Hits Arabidopsis: AT3G53170.1 (40%) Plant ESTs: Brassica napus (88%) Model Genomes:   None Subcellular Localization TargetP Targeted to Chloroplasts SUBA

Mutant Information Allele Symbol emb 2750-1 emb 2750-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Myouga / Riken Mutant Line Number 66280 RATM13-4177-1 Ecotype Columbia Nossen Genetic Background qrt   ABRC Stock CS16224   Mutagen Treatment T-DNA Transposon Insertion Mutant Yes   Details Yes   Details Flanking Sequence Details  (Help)   Location of Mutation Exon 2   Terminal Phenotype Cotyledon  Details Globular Nomarski Phenotype Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release September, 2003 December, 2010 Seed Color White to Pale Yellow   Embryo Color White to Pale Yellow   Percent Mutant Seeds 24.6 % 27.2 % Chi - Square 0.1 0.5 Seeds Counted 1038 250 Percent Top Half 47.8 %   Chi - Square 0.5   Average Seed Length 510 ± 70 µm   Average Embryo Length 310 ± 80 µm