SeedGenes Profile: EMB 2757

Gene Information Gene Symbol EMB 2757 Chromosome Locus At4g29860   Alleles in Database 4   See List OR Show All Identity Confidence Confirmed Alias Symbol TAN (J. Harada) Sequence Identity Gene Class Embryo Defective Predicted Function Uncertain Function Details WD-40 repeat protein Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Border Recovery Meinke / Syngenta (1 allele) Molecular Complementation J. Harada (1 allele) Border Recovery J. Harada (1 allele) Border Recovery Harada / Salk (1 allele) Database Release September, 2004 Predicted Sequences Gene: 4099 nucleotides mRNA: 2011 nucleotides Protein:   386 amino acids cDNA Status FLcDNA Available: BX826964 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (84%) Model Genomes:   None Subcellular Localization TargetP Targeted to Chloroplasts SUBA

Mutant Information Allele Symbol emb 2757-1 emb 2757-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Syngenta Mutant Line Number 75602 46177 Ecotype Columbia Columbia Genetic Background qrt   ABRC Stock     Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence Details  (Help)   Location of Mutation Intron 7 to Exon 8   Terminal Phenotype Cotyledon  Details Cotyledon  Details Nomarski Phenotype   Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release September, 2004 September, 2004 Seed Color Pale Yellow Pale Yellow Embryo Color Pale Yellow Pale Yellow Percent Mutant Seeds 26.2 % 26.2 % Chi - Square 0.7 0.8 Seeds Counted 901 1112 Percent Top Half 53.4 % 49.5 % Chi - Square 1.1 <0.1 Average Seed Length 540 ± 70 µm 580 ± 70 µm Average Embryo Length 470 ± 80 µm 450 ± 80 µm