SeedGenes Profile: EMB 2776

Gene Information Gene Symbol EMB 2776 Chromosome Locus At2g41500   Alleles in Database 3   See List OR Show All Identity Confidence Confirmed Alias Symbol LIS (U. Grossniklaus) Sequence Identity PRP 4 (S. Hoth) Sequence Identity Gene Class Embryo Defective Predicted Function U4/U6 snRNP (Prp4) Function Details Proposed role in pre-mRNA splicing Gene Evidence Molecular Complementation S. Hoth (1 allele) Border Recovery Meinke; Grossniklaus; Hoth / Sal (1 allele) Sequenced Mutant Allele U. Grossniklaus (1 allele) Molecular Complementation U. Grossniklaus (1 allele) Border Recovery Hoth / WiscDsLox (1 allele) Database Release October, 2007 Predicted Sequences Gene: 3297 nucleotides mRNA: 2060 nucleotides Protein:   554 amino acids cDNA Status FLcDNA Available: AK229530 Top BLAST Hits Arabidopsis: AT2G05720.1 (50%) Plant ESTs: Vitis vinifera (69%) Model Genomes:   Danio rerio (44%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 267102_at

Mutant Information Allele Symbol emb 2776 lis-1 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke; Hoth; Grossniklaus / Sa U. Grossniklaus Mutant Line Number SALK_070009   Ecotype Columbia Landsberg erecta Genetic Background     ABRC Stock     Mutagen Treatment T-DNA EMS Insertion Mutant Yes   Details No Flanking Sequence     Location of Mutation Intron 2 Exon 3 Terminal Phenotype Preglobular  Details Preglobular Nomarski Phenotype Allelism Evidence Reference Allele Sequence Identity Database Release October, 2007 October, 2007 Seed Color White   Embryo Color White   Percent Mutant Seeds 14.2 %   Chi - Square 53.2   Seeds Counted 863   Percent Top Half 39.8 %   Chi - Square 5.1   Average Seed Length 460 ± 110 µm   Average Embryo Length 30 ± 30 µm