SeedGenes Profile: EMB 3004

Gene Information Gene Symbol EMB 3004 Chromosome Locus At3g06350   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol MEE 32 (Sundaresan) Sequence Identity Gene Class Embryo Defective Predicted Function Dehydroquinate Dehydratase; Shikimate Dehydrogenase Function Details Chorismate biosynthesis Gene Evidence Single Border Recovery Meinke / Genoplante (1 allele) Border Recovery Singh / EXOTIC (1 allele) Database Release September, 2004 Predicted Sequences Gene: 3450 nucleotides mRNA: 2096 nucleotides Protein:   603 amino acids cDNA Status FLcDNA Available: BX823611 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (88%) Model Genomes:   None Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 258908_at

Mutant Information Allele Symbol emb 3004-1 emb 3004-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Genoplante Sing / EXOTIC Mutant Line Number FBU3 GT544254 Ecotype Wassilewskija   Genetic Background     ABRC Stock     Mutagen Treatment T-DNA Transposon Insertion Mutant Yes   Details Yes   Details Flanking Sequence     Location of Mutation 5` UTR to Exon 1 Exon 2 Terminal Phenotype Cotyledon  Details Not Available Nomarski Phenotype   Images Allelism Evidence Reference Allele Sequence Identity Database Release September, 2004 October, 2007 Seed Color Pale Yellow   Embryo Color Pale Yellow   Percent Mutant Seeds 24.6 %   Chi - Square 0.1   Seeds Counted 1017   Percent Top Half 56.0 %   Chi - Square 3.6   Average Seed Length 460 ± 60 µm   Average Embryo Length 140 ± 100 µm