SeedGenes Profile: HISN 3

Gene Information Gene Symbol HISN 3 Chromosome Locus At2g36230   Alleles in Database 3   See List OR Show All Identity Confidence Confirmed Alias Symbol APG 10 (K. Shinozaki) Sequence Identity Gene Class Embryo Defective Predicted Function Histidine Biosynthesis Function Details Catalyzes the third step in histidine biosynthesis Gene Evidence Border Recovery Shinozaki / Riken (1 allele) Single Border Recovery Meinke / Salk (2 alleles) Database Release October, 2007 Predicted Sequences Gene: 1845 nucleotides mRNA: 1128 nucleotides Protein:   304 amino acids cDNA Status FLcDNA Available: AB006139 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (91%) Model Genomes:   Schizosaccharomyces pombe (50%) Subcellular Localization TargetP Targeted to Mitochondria SUBA mRNA Expression Data NASCArray Spot History for 263902_at

Mutant Information Allele Symbol hisn 3-1 hisn 3-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Salk Meinke / Salk Mutant Line Number SALK_041416 SALK_097704 Ecotype Columbia Columbia Genetic Background     ABRC Stock     Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence     Location of Mutation Exon 2 Intron 4 Terminal Phenotype Preglobular  Details Preglobular  Details Nomarski Phenotype Preglobular  Images Preglobular  Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release October, 2007 October, 2007 Seed Color White White Embryo Color White White Percent Mutant Seeds 21.7 % 20.7 % Chi - Square 12.8 20.6 Seeds Counted 2168 2092 Percent Top Half 72.9 % 70.4 % Chi - Square 99.3 72.3 Average Seed Length 270 ± 80 µm 280 ± 80 µm Average Embryo Length 20 ± 10 µm 20 ± 10 µm