OSU Syngenta vbi

SeedGenes Profile: HISN 4

Gene Information
Gene Symbol HISN 4
Chromosome Locus At4g26900   tair
Alleles in Database 3   See List OR Show All
Identity Confidence Confirmed
Alias Symbol  
Gene Class Embryo Defective
Predicted Function Histidine Biosynthesis
Function Details Catalyzes the fourth step in histidine biosynthesis
Gene Evidence
Single Border Recovery
Meinke / Salk (2 alleles)
Single Border Recovery
Meinke / SAIL (1 allele)
Database Release October, 2007
Predicted Sequences
Gene:4378 nucleotides
mRNA: 2062 nucleotides
Protein:  592 amino acids
cDNA Status FLcDNA Available: AK118498
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Brassica napus (92%)
Model Genomes:  Neurospora crassa (53%)
Subcellular Localization TargetP Targeted to Chloroplasts
SUBA
mRNA Expression Data NASCArray Spot History for 253921_at
    
Mutant Information
Allele Symbol hisn 4-1hisn 4-2
Mutant Class Embryo DefectiveEmbryo Defective
Source of Mutant Meinke / SalkMeinke / Salk
Mutant Line Number SALK_041907SALK_002283
Ecotype ColumbiaColumbia
Genetic Background   
ABRC Stock   
Mutagen Treatment T-DNAT-DNA
Insertion Mutant Yes   DetailsYes   Details
Flanking Sequence   
Location of Mutation Exon 1Exon 15
Terminal Phenotype Preglobular  DetailsPreglobular  Details
Nomarski Phenotype Preglobular  ImagesEarly Globular  Images
Allelism Evidence Reference AlleleComplementation Test; Sequence Identity
Database Release October, 2007October, 2007
Seed Color WhiteWhite
Embryo Color WhiteWhite
Percent Mutant Seeds 21.8 %23.4 %
Chi - Square 10.82.8
Seeds Counted 20592114
Percent Top Half 64.7 %65.8 %
Chi - Square 38.749.8
Average Seed Length 380 ± 110 µm330 ± 110 µm
Average Embryo Length 20 ± 20 µm20 ± 20 µm