SeedGenes Profile: ISE 2

Gene Information Gene Symbol ISE 2 Chromosome Locus At1g70070   Alleles in Database 3   See List OR Show All Identity Confidence Confirmed Alias Symbol EMB 25 (D. Meinke) Sequence Identity PDE 317 (D. Meinke) Sequence Identity EMB 25 (D. Meinke) Complemetation Test PDE 317 (D. Meinke) Complemetation Test Gene Class Embryo Defective Predicted Function DEAD Box Helicase Function Details Putative RNA binding protein; Plasmodesmata function Gene Evidence Sequenced Mutant Allele P. Zambryski (2 alleles) Double Border Recovery Meinke / Syngenta (1 allele) Database Release September, 2003 Predicted Sequences Gene: 4181 nucleotides mRNA: 3564 nucleotides Protein:   1171 amino acids cDNA Status EST Identified Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Rice (Oryza sativa) (66%) Model Genomes:   Synechocystis (32%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 264748_at

Mutant Information Allele Symbol pde 317 Mutant Class Seed Pigment Source of Mutant Meinke / Syngenta Mutant Line Number 73215 Ecotype Columbia Genetic Background qrt ABRC Stock CS16227 Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence Details  (Help) Location of Mutation Exon 6 Terminal Phenotype Pigment Defective Nomarski Phenotype   Allelism Evidence Complementation Test; Sequence Identity Database Release September, 2003 Seed Color White Embryo Color White Percent Mutant Seeds 29.0 % Chi - Square 4.7 Seeds Counted 563 Percent Top Half 46.6 % Chi - Square 0.7 Average Seed Length   Average Embryo Length