OSU Syngenta vbi

SeedGenes Profile: KNF

Gene Information
Gene Symbol KNF
Chromosome Locus At1g67490   tair
Alleles in Database 3   See List OR Show All
Identity Confidence Confirmed
Alias Symbol GCS (L. Lepiniec) Complemetation Test
Gene Class Embryo Defective
Predicted Function Alpha Glucosidase I
Function Details Early step in N-glycan trimming of glycoproteins
Gene Evidence
Molecular Complementation
L. Lepiniec (1 allele)
Double Border Recovery
L. Lepiniec (2 alleles)
Sequenced Mutant Allele
C. Somerville (1 allele)
Database Release March, 2002
Predicted Sequences
Gene:6146 nucleotides
mRNA: 2756 nucleotides
Protein:  852 amino acids
cDNA Status FLcDNA Available: AK118927
Top BLAST Hits
Arabidopsis: AT1G24320.1 (74%)
Plant ESTs: Rice (Oryza sativa) (59%)
Model Genomes:  Danio rerio (42%)
Subcellular Localization TargetP Targeted to Mitochondria
SUBA
mRNA Expression Data NASCArray Spot History for 264228_at
    
Mutant Information
Allele Symbol knf-14gcs 1-1
Mutant Class Embryo DefectiveEmbryo Defective
Source of Mutant C. SomervilleLepiniec / Versailles
Mutant Line Number   
Ecotype Landsberg erectaWassilewskija
Genetic Background   
ABRC Stock CS6119 
Mutagen Treatment EMST-DNA
Insertion Mutant NoYes   Details
Flanking Sequence   
Location of Mutation Intron 2Intron 8
Terminal Phenotype Cotyledon  DetailsCotyledon
Nomarski Phenotype   Images
Allelism Evidence Reference AlleleSequence Identity
Database Release March, 2002March, 2002
Seed Color Green 
Embryo Color Green 
Percent Mutant Seeds 15.7 % 
Chi - Square 46.2 
Seeds Counted 1005 
Percent Top Half 60.1 % 
Chi - Square 6.5 
Average Seed Length 470 ± 50 µm 
Average Embryo Length 250 ± 50 µm