SeedGenes Profile: PDE 326

Gene Information Gene Symbol PDE 326 Chromosome Locus At3g03710   Alleles in Database 4   See List OR Show All Identity Confidence Confirmed Alias Symbol RIF 10 (M. Rodgriguez-C) Sequence Identity Gene Class Seed Pigment Predicted Function Plastid Polynucleotide Phosphorylase Function Details Proposed role in 23S rRNA 3' end processing Gene Evidence Border Recovery Meinke / Salk (2 alleles) Border Recovery Rodriguez-Concepcion / Weigel (1 allele) Border Recovery Rodriguez-Concepcion / Salk (1 allele) Database Release December, 2010 Predicted Sequences Gene: 5797 nucleotides mRNA: 3103 nucleotides Protein:   922 amino acids cDNA Status FLcDNA Available: AJ252123 Top BLAST Hits Arabidopsis: AT5G14580.1 (42%) Plant ESTs: Rice (Oryza sativa) (73%) Model Genomes:   Escherichia coli (40%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 259344_at

Mutant Information Allele Symbol pde 326-1 pde 326-2 Mutant Class Seed Pigment Seed Pigment Source of Mutant Meinke / Salk Meinke / Salk Mutant Line Number SALK_070705 SALK_037353 Ecotype Columbia Columbia Genetic Background     ABRC Stock     Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence     Location of Mutation Exon 12 Intron 5 Terminal Phenotype Pigment Defective  Details Pigment Defective  Details Nomarski Phenotype Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release December, 2010 December, 2010 Seed Color Pale Green Pale Green Embryo Color Pale Green Pale Green Percent Mutant Seeds 27.1 % 27.9 % Chi - Square 0.6 1.0 Seeds Counted 251 222 Percent Top Half 48.5 % 59.7 % Chi - Square 0.1 2.3 Average Seed Length 550 ± 0 µm 550 ± 0 µm Average Embryo Length 400 ± 0 µm 400 ± 0 µm