SeedGenes Profile: SUS 1

Gene Information Gene Symbol SUS 1 Chromosome Locus At1g01040   Alleles in Database 7   See List OR Show All Identity Confidence Confirmed Alias Symbol SIN 1 (A. Ray) Sequence Identity CAF (S. Jacobsen) Sequence Identity DCL 1 (A. Ray) Alternate Name EMB 76 (D. Meinke) Alternate Name Gene Class Embryo Defective Predicted Function RNA Helicase Function Details Similar in sequence to a Drosophila protein (Dicer) involved in cleavage of dsRNAs and gene silencing during development Gene Evidence Sequenced Mutant Allele A. Ray (1 allele) Double Border Recovery Meinke / Syngenta (4 alleles) Database Release March, 2002 Predicted Sequences Gene: 8082 nucleotides mRNA: 6251 nucleotides Protein:   1909 amino acids cDNA Status FLcDNA Available: AF292940 Top BLAST Hits Arabidopsis: AT3G43920.1 (37%) Plant ESTs: Medicago truncatula (70%) Model Genomes:   Caenorhabditis elegans (31%) Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 261584_at

Mutant Information Allele Symbol sus 1-1 Mutant Class Embryo Defective Source of Mutant Meinke / Feldmann Mutant Line Number 76 Ecotype Wassilewskija Genetic Background   ABRC Stock CS84713 Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence   Location of Mutation Exon 5 Terminal Phenotype Globular  Details Nomarski Phenotype   Images Allelism Evidence Complementation Test; Sequence Identity Database Release March, 2002 Seed Color Pale Yellow Embryo Color Pale Yellow Percent Mutant Seeds 26.7 % Chi - Square 1.6 Seeds Counted 1026 Percent Top Half 51.1 % Chi - Square 0.1 Average Seed Length 490 ± 40 µm Average Embryo Length 130 ± 40 µm