SeedGenes Profile: SUS 1

Gene Information Gene Symbol SUS 1 Chromosome Locus At1g01040   Alleles in Database 7   See List OR Show All Identity Confidence Confirmed Alias Symbol SIN 1 (A. Ray) Sequence Identity CAF (S. Jacobsen) Sequence Identity DCL 1 (A. Ray) Alternate Name EMB 76 (D. Meinke) Alternate Name Gene Class Embryo Defective Predicted Function RNA Helicase Function Details Similar in sequence to a Drosophila protein (Dicer) involved in cleavage of dsRNAs and gene silencing during development Gene Evidence Sequenced Mutant Allele A. Ray (1 allele) Double Border Recovery Meinke / Syngenta (4 alleles) Database Release March, 2002 Predicted Sequences Gene: 8082 nucleotides mRNA: 6251 nucleotides Protein:   1909 amino acids cDNA Status FLcDNA Available: AF292940 Top BLAST Hits Arabidopsis: AT3G43920.1 (37%) Plant ESTs: Medicago truncatula (70%) Model Genomes:   Caenorhabditis elegans (31%) Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 261584_at

Mutant Information Allele Symbol sus 1-4 sus 1-5 sus 1-6 sus 1-7 sus 1-1 sus 1-8 sus 1-9 Mutant Class Embryo Defective Embryo Defective Embryo Defective Embryo Defective Embryo Defective Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Syngenta Meinke / Syngenta Meinke / Syngenta Meinke / Feldmann Meinke / Genoplante Meinke / Genoplante Mutant Line Number 9156 12727 16360 33842 76 DYI105 EXJ1 Ecotype Columbia Columbia Uncertain Columbia Wassilewskija Wassilewskija Wassilewskija Genetic Background               ABRC Stock CS16068 CS16069 CS16070 CS16071 CS84713 CS24092 CS24094 Mutagen Treatment T-DNA T-DNA T-DNA T-DNA T-DNA T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Yes   Details Yes   Details Yes   Details Yes   Details Yes   Details Flanking Sequence Details  (Help) Details Details Details       Location of Mutation Exon 15 Exon 1 Exon 19 to 3' UTR Exon 2 Exon 5 Exon 19 to Intron 19 Exon 2 Terminal Phenotype Globular  Details Globular  Details Globular  Details Globular  Details Globular  Details Globular  Details Globular  Details Nomarski Phenotype   Images   Images   Images   Images   Images   Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Complementation Test; Sequence Identity Complementation Test; Sequence Identity Complementation Test; Sequence Identity Complementation Test; Sequence Identity Complementation Test; Sequence Identity Database Release March, 2002 March, 2002 March, 2002 March, 2002 March, 2002 March, 2004 March, 2004 Seed Color White to Pale Yellow Pale Yellow Pale Yellow Pale Yellow Pale Yellow Pale Green Pale Yellow Embryo Color White to Pale Yellow Pale Yellow Pale Yellow Pale Yellow Pale Yellow Pale Green Pale Yellow Percent Mutant Seeds 24.5 % 26.5 % 28.1 % 23.7 % 26.7 % 26.2 % 25.8 % Chi - Square 0.1 1.5 5.2 0.8 1.6 0.8 0.2 Seeds Counted 1064 1210 992 924 1026 1033 633 Percent Top Half 54.0 % 51.7 % 53.8 % 56.2 % 51.1 % 51.6 % 60.1 % Chi - Square 1.7 0.4 1.6 3.3 0.1 0.6 6.7 Average Seed Length 470 ± 40 µm 530 ± 40 µm 480 ± 30 µm 510 ± 50 µm 490 ± 40 µm 490 ± 70 µm 510 ± 70 µm Average Embryo Length 50 ± 10 µm 140 ± 50 µm 130 ± 50 µm 150 ± 50 µm 130 ± 40 µm 140 ± 60 µm 90 ± 40 µm