OSU Syngenta vbi

SeedGenes Profile: CH 42

Gene Information
Gene Symbol CH 42
Chromosome Locus At4g18480   tair
Alleles in Database 2   See List OR Show All
Identity Confidence Confirmed
Alias Symbol PDE 314 (D. Meinke) Alternate Name
Gene Class Seed Pigment
Predicted Function Magnesium Chelatase (CHLI)
Function Details First committed step in chlorophyll biosynthesis; Insertion of magnesium into protoporphyrin IX
Gene Evidence
Sequenced Mutant Allele
B. Pogson (1 allele)
Double Border Recovery
Meinke / Syngenta (1 allele)
Database Release September, 2002
Predicted Sequences
Gene:1798 nucleotides
mRNA: 1608 nucleotides
Protein:  424 amino acids
cDNA Status FLcDNA Available: AY093192
Top BLAST Hits
Arabidopsis: AT5G45930.1 (83%)
Plant ESTs: Brassica napus (94%)
Model Genomes:  Synechocystis (73%)
Subcellular Localization TargetP Targeted to Chloroplasts
SUBA
mRNA Expression Data NASCArray Spot History for 254623_at
    
Mutant Information
Allele Symbol ch 42-1ch 42-1S
Mutant Class Seed PigmentSeed Pigment
Source of Mutant J. RelichovaMeinke / Syngenta
Mutant Line Number  99807
Ecotype UncertainColumbia
Genetic Background   
ABRC Stock CS44CS16119
Mutagen Treatment IrradiationT-DNA
Insertion Mutant NoYes   Details
Flanking Sequence  Details  (Help)
Location of Mutation Exon 3Intron 2
Terminal Phenotype Pigment DefectivePigment Defective
Nomarski Phenotype
Allelism Evidence Reference AlleleSequence Identity
Database Release September, 2002September, 2002
Seed Color Pale YellowPale Yellow
Embryo Color Pale YellowPale Yellow
Percent Mutant Seeds 26.3 %23.8 %
Chi - Square 0.90.7
Seeds Counted 923952
Percent Top Half 49.8 %49.8 %
Chi - Square <0.1<0.1
Average Seed Length   
Average Embryo Length