SeedGenes Profile: EMB 1629

Gene Information Gene Symbol EMB 1629 Chromosome Locus At5g57930   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol APO 2 (J. Meurer) Sequence Identity Gene Class Embryo Defective Predicted Function Accumulation of Iron-Sulfur Complexes in Chloroplasts Function Details None Gene Evidence Molecular Complementation D. Meinke (1 allele) Double Border Recovery Meinke / Syngenta (1 allele) Single Border Recovery Meinke / Salk (1 allele) Database Release September, 2002 Predicted Sequences Gene: 1890 nucleotides mRNA: 1557 nucleotides Protein:   443 amino acids cDNA Status FLcDNA Available: BX831804 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Populus (68%) Model Genomes:   None Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 247889_at

Mutant Information Allele Symbol emb 1629-1 emb 1629-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Salk Mutant Line Number 31895 SALK_101220 Ecotype Columbia Columbia Genetic Background     ABRC Stock CS16135 CS24069 Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence Details  (Help)   Location of Mutation Exon 3 Exon 3 Terminal Phenotype Globular  Details Globular  Details Nomarski Phenotype   Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release September, 2002 March, 2004 Seed Color White White Embryo Color White White Percent Mutant Seeds 24.6 % 25.7 % Chi - Square 0.1 0.3 Seeds Counted 866 1070 Percent Top Half 48.4 % 49.1 % Chi - Square 0.2 0.1 Average Seed Length 560 ± 50 µm 500 ± 60 µm Average Embryo Length 60 ± 20 µm 90 ± 50 µm