SeedGenes Profile: EMB 1789

Gene Information Gene Symbol EMB 1789 Chromosome Locus At5g56930   Alleles in Database 3   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Uncertain Function Details Putative zinc finger domain Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Single Border Recovery Meinke / Salk (2 alleles) Database Release March, 2002 Predicted Sequences Gene: 2674 nucleotides mRNA: 2085 nucleotides Protein:   675 amino acids cDNA Status FLcDNA Available: BX832581 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (74%) Model Genomes:   None Subcellular Localization TargetP Targeted to Mitochondria SUBA

Mutant Information Allele Symbol emb 1789-1 emb 1789-2 emb 1789-3 Mutant Class Embryo Defective Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Salk Meinke / Salk Mutant Line Number 36637 SALK_007855 SALK_034075 Ecotype Columbia Columbia Columbia Genetic Background       ABRC Stock CS16033 CS24074 CS24075 Mutagen Treatment T-DNA T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Yes   Details Flanking Sequence Details  (Help)     Location of Mutation Exon 2 Exon 2 Exon 7 Terminal Phenotype Globular  Details Globular  Details Globular  Details Nomarski Phenotype   Images   Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Complementation Test; Sequence Identity Database Release March, 2002 March, 2004 March, 2004 Seed Color White to Pale Yellow Pale Yellow Pale Yellow Embryo Color White to Pale Yellow Pale Yellow Pale Yellow Percent Mutant Seeds 28.0 % 21.6 % 22.4 % Chi - Square 4.6 6.5 3.8 Seeds Counted 935 1056 1075 Percent Top Half 49.2 % 44.3 % 46.1 % Chi - Square 0.1 3.0 1.4 Average Seed Length 430 ± 50 µm 510 ± 50 µm 510 ± 50 µm Average Embryo Length 20 ± 20 µm 80 ± 40 µm 80 ± 40 µm