OSU Syngenta vbi

SeedGenes Profile: EMB 1873

Gene Information
Gene Symbol EMB 1873
Chromosome Locus At5g08170   tair
Alleles in Database 3   See List OR Show All
Identity Confidence Confirmed
Alias Symbol  
Gene Class Embryo Defective
Predicted Function Agmatine Iminohydrolase
Function Details Polyamine biosynthesis
Gene Evidence
Double Border Recovery
Meinke / Syngenta (1 allele)
Single Border Recovery
Meinke / Salk (2 alleles)
Database Release September, 2002
Predicted Sequences
Gene:2771 nucleotides
mRNA: 1538 nucleotides
Protein:  383 amino acids
cDNA Status FLcDNA Available: AK118589
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Brassica napus (89%)
Model Genomes:   None
Subcellular Localization TargetP Not Targeted to Cell Compartment
SUBA
mRNA Expression Data NASCArray Spot History for 250570_at
    
Mutant Information
Allele Symbol emb 1873-1emb 1873-2emb 1873-3
Mutant Class Embryo DefectiveEmbryo DefectiveEmbryo Defective
Source of Mutant Meinke / SyngentaMeinke / SalkMeinke / Salk
Mutant Line Number 43301SALK_138993SALK_020442
Ecotype ColumbiaColumbiaColumbia
Genetic Background    
ABRC Stock CS16128CS24077CS24078
Mutagen Treatment T-DNAT-DNAT-DNA
Insertion Mutant Yes   DetailsYes   DetailsYes   Details
Flanking Sequence Details  (Help)  
Location of Mutation Intron 1Exon 4Exon 6
Terminal Phenotype Cotyledon  DetailsCotyledon  DetailsCotyledon  Details
Nomarski Phenotype   Images  Images  Images
Allelism Evidence Reference AlleleComplementation Test; Sequence IdentityComplementation Test; Sequence Identity
Database Release September, 2002March, 2004March, 2004
Seed Color Pale YellowPale GreenPale Green
Embryo Color Pale YellowPale GreenPale Green
Percent Mutant Seeds 26.6 %25.7 %26.0 %
Chi - Square 1.40.30.5
Seeds Counted 101111371039
Percent Top Half 52.4 %58.9 %50.7 %
Chi - Square 0.69.20.1
Average Seed Length 470 ± 50 µm500 ± 50 µm520 ± 60 µm
Average Embryo Length 260 ± 80 µm370 ± 50 µm390 ± 50 µm