OSU Syngenta vbi

SeedGenes Profile: EMB 1899

Gene Information
Gene Symbol EMB 1899
Chromosome Locus At5g03800   tair
Alleles in Database 4   See List OR Show All
Identity Confidence Confirmed
Alias Symbol EMB 175 (D. Vernon) Sequence Identity
EMB 166 (D. Meinke) Alternate Name
Gene Class Embryo Defective
Predicted Function PPR Protein
Function Details Processing of organellar mRNAs
Gene Evidence
Single Border Recovery
Vernon / Feldmann (1 allele)
Double Border Recovery
Meinke / Syngenta (2 alleles)
Database Release March, 2002
Predicted Sequences
Gene:2807 nucleotides
mRNA: 2807 nucleotides
Protein:  896 amino acids
cDNA Status FLcDNA Available: AK117204
Top BLAST Hits
Arabidopsis: AT5G16860.1 (31%)
Plant ESTs: Rice (Oryza sativa) (44%)
Model Genomes:   None
Subcellular Localization TargetP Targeted to Chloroplasts
SUBA
mRNA Expression Data NASCArray Spot History for 250893_at
    
Mutant Information
Allele Symbol emb 1899-1emb 1899-2emb 175-1emb 175-2
Mutant Class Embryo DefectiveEmbryo DefectiveEmbryo DefectiveEmbryo Defective
Source of Mutant Meinke / SyngentaMeinke / SyngentaVernon / FeldmannMeinke / Feldmann
Mutant Line Number 439517398466795766b / emb 166
Ecotype ColumbiaColumbiaWassilewskijaWassilewskija
Genetic Background  qrt  
ABRC Stock CS16038CS16039  
Mutagen Treatment T-DNAT-DNAT-DNAT-DNA
Insertion Mutant Yes   DetailsYes   DetailsYes   DetailsYes   Details
Flanking Sequence Details  (Help)Details  
Location of Mutation Exon 1Exon 1  
Terminal Phenotype Globular  DetailsGlobular  DetailsGlobularGlobular
Nomarski Phenotype   Images  Images
Allelism Evidence Reference AlleleComplementation Test; Sequence IdentityComplementation Test; Sequence IdentityComplementation Test; Sequence Identity
Database Release March, 2002March, 2002December, 2010December, 2010
Seed Color WhiteWhite  
Embryo Color WhiteWhite  
Percent Mutant Seeds 25.2 %25.6 %  
Chi - Square <0.10.2  
Seeds Counted 9471200  
Percent Top Half 54.4 %48.9 %  
Chi - Square 1.80.2  
Average Seed Length 430 ± 60 µm490 ± 40 µm  
Average Embryo Length 80 ± 40 µm70 ± 20 µm