SeedGenes Profile: EMB 1989

Gene Information Gene Symbol EMB 1989 Chromosome Locus At4g21710   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol NRPB 2 (C. Pikaard) Sequence Identity Gene Class Embryo Defective Predicted Function RNA Polymerase II Subunit Function Details Transcription Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Database Release September, 2003 Predicted Sequences Gene: 6744 nucleotides mRNA: 3794 nucleotides Protein:   1188 amino acids cDNA Status FLcDNA Available: Z19120 Top BLAST Hits Arabidopsis: AT5G45140.1 (36%) Plant ESTs: Tomato (Lycopersicon esculentum) (94%) Model Genomes:   Homo sapiens (67%) Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 254368_at

Mutant Information Allele Symbol emb 1989-1 emb 1989-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Syngenta Mutant Line Number 47489 34522 Ecotype Columbia Columbia Genetic Background     ABRC Stock CS16201 CS16202 Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence Details  (Help)   Location of Mutation Upstream to Intron 1   Terminal Phenotype Preglobular  Details Preglobular  Details Nomarski Phenotype   Images Globular  Images Allelism Evidence Reference Allele Complementation Test Database Release September, 2003 September, 2003 Seed Color White to Pale Yellow Pale Yellow Embryo Color White to Pale Yellow Pale Yellow Percent Mutant Seeds 20.3 % 28.5 % Chi - Square 10.4 6.7 Seeds Counted 869 995 Percent Top Half 44.3 % 51.0 % Chi - Square 2.3 0.1 Average Seed Length 520 ± 70 µm 490 ± 60 µm Average Embryo Length 20 ± 10 µm 20 ± 20 µm