SeedGenes Profile: EMB 2083

Gene Information Gene Symbol EMB 2083 Chromosome Locus At3g16290   Alleles in Database 3   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function FtsH Protease (AAA ATPase) Function Details Chloroplast ATP-dependent zinc metalloprotease Gene Evidence Double Border Recovery Meinke / Syngenta (3 alleles) Database Release March, 2003 Predicted Sequences Gene: 3925 nucleotides mRNA: 2747 nucleotides Protein:   876 amino acids cDNA Status FLcDNA Available: BX823945 Top BLAST Hits Arabidopsis: AT1G50250.1 (38%) Plant ESTs: Vitis vinifera (86%) Model Genomes:   Synechocystis (41%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 258048_at

Mutant Information Allele Symbol emb 2083-2 emb 2083-3 emb 2083-4 Mutant Class Embryo Defective Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Syngenta Meinke / Syngenta Mutant Line Number 117811 98346 99365 Ecotype Columbia Columbia Columbia Genetic Background qrt     ABRC Stock CS16167 CS16208 CS16209 Mutagen Treatment T-DNA T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Yes   Details Flanking Sequence Details  (Help) Details Details Location of Mutation Intron 3 to Exon 4 Exon 1 Upstream to Exon 1 Terminal Phenotype Cotyledon  Details Transition  Details Transition  Details Nomarski Phenotype   Images   Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Complementation Test; Sequence Identity Database Release March, 2003 March, 2003 March, 2003 Seed Color White White White Embryo Color White White White Percent Mutant Seeds 21.6 % 23.5 % 24.3 % Chi - Square 6.5 1.0 0.3 Seeds Counted 1042 879 1241 Percent Top Half 54.2 % 55.1 % 52.3 % Chi - Square 1.6 2.1 0.7 Average Seed Length 500 ± 40 µm 530 ± 50 µm 520 ± 30 µm Average Embryo Length 140 ± 70 µm 110 ± 50 µm 100 ± 40 µm