OSU Syngenta vbi

SeedGenes Profile: EMB 2261

Gene Information
Gene Symbol EMB 2261
Chromosome Locus At3g49170   tair
Alleles in Database 2   See List OR Show All
Identity Confidence Confirmed
Alias Symbol  
Gene Class Embryo Defective
Predicted Function PPR Protein
Function Details Processing of organellar mRNAs
Gene Evidence
Double Border Recovery
Meinke / Syngenta (1 allele)
Single Border Recovery
Vernon / Salk (1 allele)
Database Release March, 2002
Predicted Sequences
Gene:2700 nucleotides
mRNA: 2606 nucleotides
Protein:  850 amino acids
cDNA Status FLcDNA Available: AY864347
Top BLAST Hits
Arabidopsis: AT4G13650.1 (35%)
Plant ESTs: Rice (Oryza sativa) (48%)
Model Genomes:   None
Subcellular Localization TargetP Targeted to Chloroplasts
Mutant Information
Allele Symbol emb 2261-1emb 2261-2
Mutant Class Embryo DefectiveEmbryo Defective
Source of Mutant Meinke / SyngentaVernon / Salk
Mutant Line Number 74313SALK_024975
Ecotype ColumbiaColumbia
Genetic Background qrt 
ABRC Stock CS16045 
Mutagen Treatment T-DNAT-DNA
Insertion Mutant Yes   DetailsYes   Details
Flanking Sequence Details  (Help) 
Location of Mutation Exon 1Exon 1
Terminal Phenotype Cotyledon  DetailsCotyledon
Nomarski Phenotype
Allelism Evidence Reference AlleleComplementation Test; Sequence Identity
Database Release March, 2002December, 2010
Seed Color White 
Embryo Color White 
Percent Mutant Seeds 27.0 % 
Chi - Square 2.1 
Seeds Counted 1004 
Percent Top Half 51.3 % 
Chi - Square 0.1 
Average Seed Length 470 ± 50 µm 
Average Embryo Length 230 ± 90 µm