OSU Syngenta vbi

SeedGenes Profile: EMB 2296

Gene Information
Gene Symbol EMB 2296
Chromosome Locus At2g18020   tair
Alleles in Database 2   See List OR Show All
Identity Confidence Confirmed
Alias Symbol  
Gene Class Embryo Defective
Predicted Function Ribosomal Protein L8/L2
Function Details Translation in mitochondria or cytoplasm
Gene Evidence
Double Border Recovery
Meinke / Syngenta (2 alleles)
Database Release March, 2002
Predicted Sequences
Gene:1202 nucleotides
mRNA: 969 nucleotides
Protein:  258 amino acids
cDNA Status FLcDNA Available: BX820387
Top BLAST Hits
Arabidopsis: AT4G36130.1 (96%)
Plant ESTs: Brassica napus (96%)
Model Genomes:  Drosophila melanogaster (72%)
Subcellular Localization TargetP Targeted to Mitochondria
SUBA
mRNA Expression Data NASCArray Spot History for 265805_s_at
    
Mutant Information
Allele Symbol emb 2296-1emb 2296-2
Mutant Class Embryo DefectiveEmbryo Defective
Source of Mutant Meinke / SyngentaMeinke / Syngenta
Mutant Line Number 7886186141
Ecotype ColumbiaColumbia
Genetic Background qrt 
ABRC Stock CS16050CS16051
Mutagen Treatment T-DNAT-DNA
Insertion Mutant Yes   DetailsYes   Details
Flanking Sequence Details  (Help)Details
Location of Mutation Exon 2Exon 2 to 3' UTR
Terminal Phenotype Globular  DetailsGlobular  Details
Nomarski Phenotype   Images  Images
Allelism Evidence Reference AlleleComplementation Test; Sequence Identity
Database Release March, 2002March, 2002
Seed Color WhiteWhite
Embryo Color WhiteWhite
Percent Mutant Seeds 30.0 %21.1 %
Chi - Square 13.17.7
Seeds Counted 991953
Percent Top Half 54.5 %55.7 %
Chi - Square 2.42.6
Average Seed Length 450 ± 40 µm440 ± 30 µm
Average Embryo Length 60 ± 30 µm110 ± 50 µm