SeedGenes Profile: EMB 2394

Gene Information Gene Symbol EMB 2394 Chromosome Locus At1g05190   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Chloroplast 50S Ribosomal Protein L6 Function Details Translation in chloroplast Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Border Recovery Myouga / Riken (1 allele) Database Release March, 2003 Predicted Sequences Gene: 1487 nucleotides mRNA: 935 nucleotides Protein:   223 amino acids cDNA Status FLcDNA Available: AY085715 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (94%) Model Genomes:   Synechocystis (50%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 264575_at

Mutant Information Allele Symbol emb 2394-1 emb 2394-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Myouga / Riken Mutant Line Number 82754 RATM54-1238-1 Ecotype Columbia Nossen Genetic Background     ABRC Stock CS16176   Mutagen Treatment T-DNA Transposon Insertion Mutant Yes   Details Yes   Details Flanking Sequence Details  (Help)   Location of Mutation Intron 1   Terminal Phenotype Globular  Details Preglobular Nomarski Phenotype Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release March, 2003 December, 2010 Seed Color White   Embryo Color White   Percent Mutant Seeds 24.8 % 24.0 % Chi - Square <0.1 0.2 Seeds Counted 1179 295 Percent Top Half 48.6 %   Chi - Square 0.2   Average Seed Length 480 ± 50 µm   Average Embryo Length 120 ± 60 µm