SeedGenes Profile: EMB 2742

Gene Information Gene Symbol EMB 2742 Chromosome Locus At3g12670   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function CTP Synthase (UTP-Ammonia Ligase) Function Details Ribonucleotide metabolism Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Single Border Recovery Meinke / Syngenta (1 allele) Database Release March, 2003 Predicted Sequences Gene: 4149 nucleotides mRNA: 2056 nucleotides Protein:   591 amino acids cDNA Status FLcDNA Available: AY093110 Top BLAST Hits Arabidopsis: AT1G30820.1 (76%) Plant ESTs: Rice (Oryza sativa) (78%) Model Genomes:   Homo sapiens (59%) Subcellular Localization TargetP Targeted to Secretory Pathway SUBA mRNA Expression Data NASCArray Spot History for 257702_at

Mutant Information Allele Symbol emb 2742-1 emb 2742-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Syngenta Mutant Line Number 126850 94848 Ecotype Columbia Columbia Genetic Background     ABRC Stock CS16190 CS16219 Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence Details  (Help) Details Location of Mutation Intron 5 Upstream Terminal Phenotype Globular  Details Globular  Details Nomarski Phenotype   Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release March, 2003 March, 2003 Seed Color Pale Yellow Pale Yellow Embryo Color Pale Yellow Pale Yellow Percent Mutant Seeds 22.4 % 22.7 % Chi - Square 3.7 2.7 Seeds Counted 1248 1011 Percent Top Half 54.8 % 55.2 % Chi - Square 2.6 2.5 Average Seed Length 500 ± 40 µm 490 ± 40 µm Average Embryo Length 20 ± 20 µm 30 ± 20 µm