SeedGenes Profile: EMB 2781

Gene Information Gene Symbol EMB 2781 Chromosome Locus At1g04950   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol AtTAF 6 (M. Kater) Sequence Identity Gene Class Embryo Defective Predicted Function TATA Box Binding Protein Function Details Regulation of transcription Gene Evidence Border Recovery Meinke / Salk (2 alleles) Database Release December, 2010 Predicted Sequences Gene: 4384 nucleotides mRNA: 2192 nucleotides Protein:   549 amino acids cDNA Status FLcDNA Available: AY050962 Top BLAST Hits Arabidopsis: AT1G54360.1 (42%) Plant ESTs: Brassica napus (90%) Model Genomes:   Schizosaccharomyces pombe (43%) Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 261126_at

Mutant Information Allele Symbol emb 2781-1 emb 2781-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Salk Meinke / Salk Mutant Line Number SALK_038879 SALK_137678 Ecotype Columbia Columbia Genetic Background     ABRC Stock     Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence     Location of Mutation Intron 10 Intron 10 Terminal Phenotype Preglobular  Details Preglobular  Details Nomarski Phenotype   Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release December, 2010 December, 2010 Seed Color White White to Pale Yellow Embryo Color White White to Pale Yellow Percent Mutant Seeds 22.4 % 25.6 % Chi - Square 0.9 <0.1 Seeds Counted 250 215 Percent Top Half 58.9 % 52.7 % Chi - Square 1.8 0.2 Average Seed Length 340 ± 60 µm 520 ± 30 µm Average Embryo Length 15 ± 0 µm 15 ± 0 µm