SeedGenes Profile: EMB 2782

Gene Information Gene Symbol EMB 2782 Chromosome Locus At5g15920   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function SMC Family Protein (MSS2) Function Details Chromosome dynamics (SMC5) Gene Evidence Border Recovery Meinke / Salk (2 alleles) Database Release December, 2010 Predicted Sequences Gene: 3877 nucleotides mRNA: 3279 nucleotides Protein:   1053 amino acids cDNA Status EST Identified Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Rice (Oryza sativa) (59%) Model Genomes:   Homo sapiens (32%) Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 246536_at

Mutant Information Allele Symbol emb 2782-1 emb 2782-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Salk Meinke / Salk Mutant Line Number SALK_107583 SALK_092081 Ecotype Columbia Columbia Genetic Background     ABRC Stock     Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence     Location of Mutation Exon 1 Exon 1 Terminal Phenotype Preglobular  Details Preglobular  Details Nomarski Phenotype   Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release December, 2010 December, 2010 Seed Color White to Pale Yellow White to Pale Yellow Embryo Color White to Pale Yellow White to Pale Yellow Percent Mutant Seeds 23.3 % 28.4 % Chi - Square 0.4 1.4 Seeds Counted 275 229 Percent Top Half 56.2 % 49.2 % Chi - Square 1 <0.1 Average Seed Length 490 ± 100 µm 490 ± 110 µm Average Embryo Length 40 ± 80 µm 30 ± 60 µm