SeedGenes Profile: EMB 2793

Gene Information Gene Symbol EMB 2793 Chromosome Locus At1g24706   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function THO/TREX Complex Component (THO2) Function Details Transcription elongation; mRNP biogenesis; genome integrity Gene Evidence Border Recovery Meinke / Salk (2 alleles) Database Release December, 2010 Predicted Sequences Gene: 13619 nucleotides mRNA: 5913 nucleotides Protein:   1804 amino acids cDNA Status EST Identified Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Rice (Oryza sativa) (53%) Model Genomes:   Homo sapiens (35%) Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 245647_at

Mutant Information Allele Symbol emb 2793-1 emb 2793-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Salk Meinke / Salk Mutant Line Number SALK_130342 SALK_072011 Ecotype Columbia Columbia Genetic Background     ABRC Stock     Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence     Location of Mutation Exon 17 Exon 15 Terminal Phenotype Cotyledon  Details Cotyledon  Details Nomarski Phenotype   Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release December, 2010 December, 2010 Seed Color Pale Green Pale Green Embryo Color Pale Green Pale Green Percent Mutant Seeds 21.6 % 23.3 % Chi - Square 1.8 0.4 Seeds Counted 292 283 Percent Top Half 50.8 % 59.1 % Chi - Square <0.1 2.2 Average Seed Length 520 ± 50 µm 470 ± 110 µm Average Embryo Length 360 ± 90 µm 260 ± 160 µm