SeedGenes Profile: PDE 318

Gene Information Gene Symbol PDE 318 Chromosome Locus At1g80770   Alleles in Database 3   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Seed Pigment Predicted Function Uncertain Function Details GTP binding protein Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Border Recovery Meinke / Salk (2 alleles) Database Release September, 2003 Predicted Sequences Gene: 2848 nucleotides mRNA: 1683 nucleotides Protein:   451 amino acids cDNA Status FLcDNA Available: AY128318 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (90%) Model Genomes:   Homo sapiens (26%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 261882_at

Mutant Information Allele Symbol pde 318-1 pde 318-2 pde 318-3 Mutant Class Seed Pigment Seed Pigment Seed Pigment Source of Mutant Meinke / Syngenta Meinke / Salk Meinke / Salk Mutant Line Number 102326 SALK_145367 SALK_048569 Ecotype Columbia Columbia Columbia Genetic Background       ABRC Stock CS16228     Mutagen Treatment T-DNA T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Yes   Details Flanking Sequence Details  (Help)     Location of Mutation Intron 7 to Exon 8 Exon 5 Exon 12 Terminal Phenotype Pigment Defective Pigment Defective Pigment Defective Nomarski Phenotype Allelism Evidence Reference Allele Complementation Test; Sequence Identity Complementation Test; Sequence Identity Database Release September, 2003 December, 2010 December, 2010 Seed Color Pale Green to Green     Embryo Color Pale Green to Green     Percent Mutant Seeds 22.7 % 23.2 % 24.0 % Chi - Square 2.6 0.7 0.1 Seeds Counted 909 396 200 Percent Top Half 54.9 %     Chi - Square 1.9     Average Seed Length       Average Embryo Length