OSU Syngenta vbi

SeedGenes Profile: PDE 318

Gene Information
Gene Symbol PDE 318
Chromosome Locus At1g80770   tair
Alleles in Database 3   See List OR Show All
Identity Confidence Confirmed
Alias Symbol  
Gene Class Seed Pigment
Predicted Function Uncertain
Function Details GTP binding protein
Gene Evidence
Double Border Recovery
Meinke / Syngenta (1 allele)
Border Recovery
Meinke / Salk (2 alleles)
Database Release September, 2003
Predicted Sequences
Gene:2848 nucleotides
mRNA: 1683 nucleotides
Protein:  451 amino acids
cDNA Status FLcDNA Available: AY128318
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Brassica napus (90%)
Model Genomes:  Homo sapiens (26%)
Subcellular Localization TargetP Targeted to Chloroplasts
SUBA
mRNA Expression Data NASCArray Spot History for 261882_at
    
Mutant Information
Allele Symbol pde 318-1pde 318-2pde 318-3
Mutant Class Seed PigmentSeed PigmentSeed Pigment
Source of Mutant Meinke / SyngentaMeinke / SalkMeinke / Salk
Mutant Line Number 102326SALK_145367SALK_048569
Ecotype ColumbiaColumbiaColumbia
Genetic Background    
ABRC Stock CS16228  
Mutagen Treatment T-DNAT-DNAT-DNA
Insertion Mutant Yes   DetailsYes   DetailsYes   Details
Flanking Sequence Details  (Help)  
Location of Mutation Intron 7 to Exon 8Exon 5Exon 12
Terminal Phenotype Pigment DefectivePigment DefectivePigment Defective
Nomarski Phenotype
Allelism Evidence Reference AlleleComplementation Test; Sequence IdentityComplementation Test; Sequence Identity
Database Release September, 2003December, 2010December, 2010
Seed Color Pale Green to Green  
Embryo Color Pale Green to Green  
Percent Mutant Seeds 22.7 %23.2 %24.0 %
Chi - Square 2.60.70.1
Seeds Counted 909396200
Percent Top Half 54.9 %  
Chi - Square 1.9  
Average Seed Length    
Average Embryo Length