SeedGenes Profile: PDE 324

Gene Information Gene Symbol PDE 324 Chromosome Locus At3g04260   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Seed Pigment Predicted Function SAP Domain Protein (PTAC3) Function Details Plastid gene expression Gene Evidence Border Recovery Meinke / Salk (2 alleles) Database Release December, 2010 Predicted Sequences Gene: 4493 nucleotides mRNA: 2950 nucleotides Protein:   913 amino acids cDNA Status EST Identified Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (88%) Model Genomes:   None Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 258573_at

Mutant Information Allele Symbol pde 324-1 pde 324-2 Mutant Class Seed Pigment Seed Pigment Source of Mutant Meinke / Salk Meinke / Salk Mutant Line Number SALK_108852 SALK_110045 Ecotype Columbia Columbia Genetic Background     ABRC Stock     Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence     Location of Mutation Intron 10 Exon 2 Terminal Phenotype Pigment Defective  Details Pigment Defective  Details Nomarski Phenotype Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release December, 2010 December, 2010 Seed Color White to Pale Yellow White to Pale Yellow Embryo Color White to Pale Yellow White to Pale Yellow Percent Mutant Seeds 25.8 % 22.4 % Chi - Square 0.1 1.1 Seeds Counted 326 295 Percent Top Half 54.8 % 53.0 % Chi - Square 0.8 0.2 Average Seed Length 550 ± 0 µm 550 ± 10 µm Average Embryo Length 500 ± 0 µm 500 ± 10 µm