SeedGenes Profile: SLP

Gene Information Gene Symbol SLP Chromosome Locus At2g28000   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Chaperonin-60a Function Details Protein folding in chloroplast; similar to prokaryotic groEL Gene Evidence Molecular Complementation R. Goldberg (1 allele) Double Border Recovery R. Goldberg (1 allele) Double Border Recovery Meinke / Syngenta (1 allele) Database Release March, 2002 Predicted Sequences Gene: 2941 nucleotides mRNA: 2120 nucleotides Protein:   586 amino acids cDNA Status FLcDNA Available: BT002441 Top BLAST Hits Arabidopsis: AT5G18820.1 (61%) Plant ESTs: Brassica napus (94%) Model Genomes:   Synechocystis (57%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 264069_at

Mutant Information Allele Symbol slp-1S slp-1 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Goldberg / Feldmann Mutant Line Number 21157   Ecotype Columbia Wassilewskija Genetic Background     ABRC Stock CS16067 CS3719 Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence Details  (Help)   Location of Mutation Exon 2 to Intron 2 Exon 7 Terminal Phenotype Cotyledon  Details Cotyledon  Details Nomarski Phenotype   Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release March, 2002 March, 2002 Seed Color White White Embryo Color White White Percent Mutant Seeds 26.6 % 24.5 % Chi - Square 1.4 0.1 Seeds Counted 1026 1077 Percent Top Half 50.2 % 58.7 % Chi - Square <0.1 4.0 Average Seed Length 490 ± 40 µm 430 ± 40 µm Average Embryo Length 260 ± 100 µm 210 ± 80 µm