OSU Syngenta vbi

SeedGenes Profile: TTN 1

Gene Information
Gene Symbol TTN 1
Chromosome Locus At3g60740   tair
Alleles in Database 3   See List OR Show All
Identity Confidence Confirmed
Alias Symbol CHO (G. Jurgens) Sequence Identity
EMB 133 (D. Meinke) Alternate Name
Gene Class Embryo Defective
Predicted Function Tubulin Folding Cofactor D
Function Details Postulated to interact with TTN5 protein to regulate B-tubulin folding and microtubule dynamics
Gene Evidence
Double Border Recovery
Meinke / Syngenta (2 alleles)
Database Release March, 2002
Predicted Sequences
Gene:6120 nucleotides
mRNA: 3944 nucleotides
Protein:  1254 amino acids
cDNA Status FLcDNA Available: AF486851
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Rice (Oryza sativa) (58%)
Model Genomes:  Danio rerio (35%)
Subcellular Localization TargetP Not Targeted to Cell Compartment
SUBA
mRNA Expression Data NASCArray Spot History for 251383_at
    
Mutant Information
Allele Symbol ttn 1-2ttn 1-3ttn 1-1
Mutant Class Embryo DefectiveEmbryo DefectiveEmbryo Defective
Source of Mutant Meinke / SyngentaMeinke / SyngentaMeinke / Feldmann
Mutant Line Number 29862230752794
Ecotype ColumbiaColumbiaWassilewskija
Genetic Background    
ABRC Stock CS16074CS16075CS84717
Mutagen Treatment T-DNAT-DNAT-DNA
Insertion Mutant Yes   DetailsYes   DetailsNo
Flanking Sequence Details  (Help)Details 
Location of Mutation Exon 2Intron 9Unknown
Terminal Phenotype Preglobular  DetailsPreglobular  DetailsPreglobular  Details
Nomarski Phenotype Preglobular  ImagesPreglobular  ImagesPreglobular  Images
Allelism Evidence Reference AlleleComplementation Test; Sequence IdentityComplementation Test
Database Release March, 2002March, 2002March, 2002
Seed Color WhiteWhiteWhite
Embryo Color WhiteWhiteWhite
Percent Mutant Seeds 22.2 %26.5 %22.1 %
Chi - Square 4.41.45.6
Seeds Counted 105411041215
Percent Top Half 55.5 %53.2 %44.8 %
Chi - Square 2.91.22.9
Average Seed Length 420 ± 70 µm460 ± 40 µm470 ± 40 µm
Average Embryo Length 20 ± 10 µm15 ± 0 µm20 ± 20 µm