SeedGenes Profile: TTN 8

Gene Information Gene Symbol TTN 8 Chromosome Locus At3g54670   Alleles in Database 3   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function SMC1 Cohesin Function Details Structural maintenance of chromosome (SMC) protein; chromosomal ATPase involved in sister chromatid cohesion and chromosome segregation in yeast and animal systems Gene Evidence Double Border Recovery Meinke / Syngenta (2 alleles) Database Release March, 2002 Predicted Sequences Gene: 8283 nucleotides mRNA: 4116 nucleotides Protein:   1238 amino acids cDNA Status FLcDNA Available: AY567966 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Rice (Oryza sativa) (57%) Model Genomes:   Homo sapiens (36%) Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 251851_at

Mutant Information Allele Symbol ttn 8-1 ttn 8-2 ttn 8-3 Mutant Class Embryo Defective Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Syngenta Meinke / Genoplante Mutant Line Number 55132 51055 FBS4 Ecotype Columbia Columbia Wassilewskija Genetic Background       ABRC Stock CS16082 CS16083 CS24097 Mutagen Treatment T-DNA T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Yes   Details Flanking Sequence Details  (Help) Details   Location of Mutation Exon 15 Exon 2 to Intron 3 Intron 16 Terminal Phenotype Preglobular  Details Preglobular  Details Preglobular  Details Nomarski Phenotype Preglobular  Images Preglobular  Images Preglobular  Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Complementation Test; Sequence Identity Database Release March, 2002 March, 2002 March, 2004 Seed Color White White Pale Green Embryo Color White White Pale Green Percent Mutant Seeds 23.3 % 24.4 % 22.7 % Chi - Square 1.6 0.2 2.7 Seeds Counted 984 1175 972 Percent Top Half 48.0 % 48.1 % 49.3 % Chi - Square 0.4 0.4 <0.1 Average Seed Length 420 ± 60 µm 420 ± 70 µm 320 ± 90 µm Average Embryo Length 20 ± 20 µm 15 ± 0 µm 15 ± 0 µm