OSU Syngenta vbi

SeedGenes Profile: BIO 1

Gene Information
Gene Symbol BIO 1
Chromosome Locus At5g57590   tair
Alleles in Database 6   See List OR Show All
Identity Confidence Confirmed
Alias Symbol BIO 3 (D. Meinke) Alternate Name
Gene Class Embryo Defective
Predicted Function 7-KAP, DAP Aminotransferase; Dethiobiotin Synthetase
Function Details Biotin synthesis in mitochondria; Bifunctional (BIO3-BIO1) protein
Gene Evidence
Experimental Data
D. Meinke (1 allele)
Double Border Recovery
Meinke / Syngenta (1 allele)
Multigene Disruption
Meinke / Syngenta (1 allele)
Border Recovery
Meinke / Riken (2 alleles)
Border Recovery
Meinke / Salk (1 allele)
Database Release March, 2002
Predicted Sequences
Gene:4263 nucleotides
mRNA: 2670 nucleotides
Protein:  833 amino acids
cDNA Status FLcDNA Available: EU089963
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Rice (Oryza sativa) (58%)
Model Genomes:   None
Subcellular Localization TargetP Targeted to Mitochondria
SUBA
mRNA Expression Data NASCArray Spot History for 247916_at
    
Mutant Information
Allele Symbol bio 1-2bio 1-3
Mutant Class Embryo DefectiveEmbryo Defective
Source of Mutant Meinke / SyngentaMeinke / Syngenta
Mutant Line Number 3617246455
Ecotype ColumbiaColumbia
Genetic Background   
ABRC Stock CS16001CS16002
Mutagen Treatment T-DNAT-DNA
Insertion Mutant Yes   DetailsYes   Details
Flanking Sequence Details  (Help)Details
Location of Mutation Exon 8Downstream to At5g57580
Terminal Phenotype Cotyledon  DetailsCotyledon  Details
Nomarski Phenotype   Images  Images
Allelism Evidence Reference AlleleComplementation Test; Sequence Identity
Database Release March, 2002March, 2002
Seed Color Pale YellowWhite
Embryo Color Pale YellowWhite
Percent Mutant Seeds 27.0 %34.8 %
Chi - Square 3.351.0
Seeds Counted 1045994
Percent Top Half 48.9 %55.8 %
Chi - Square 0.12.3
Average Seed Length 510 ± 40 µm510 ± 40 µm
Average Embryo Length 300 ± 100 µm320 ± 90 µm