SeedGenes Profile: BIO 1

Gene Information Gene Symbol BIO 1 Chromosome Locus At5g57590   Alleles in Database 6   See List OR Show All Identity Confidence Confirmed Alias Symbol BIO 3 (D. Meinke) Alternate Name Gene Class Embryo Defective Predicted Function 7-KAP, DAP Aminotransferase; Dethiobiotin Synthetase Function Details Biotin synthesis in mitochondria; Bifunctional (BIO3-BIO1) protein Gene Evidence Experimental Data D. Meinke (1 allele) Double Border Recovery Meinke / Syngenta (1 allele) Multigene Disruption Meinke / Syngenta (1 allele) Border Recovery Meinke / Riken (2 alleles) Border Recovery Meinke / Salk (1 allele) Database Release March, 2002 Predicted Sequences Gene: 4263 nucleotides mRNA: 2670 nucleotides Protein:   833 amino acids cDNA Status FLcDNA Available: EU089963 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Rice (Oryza sativa) (58%) Model Genomes:   None Subcellular Localization TargetP Targeted to Mitochondria SUBA mRNA Expression Data NASCArray Spot History for 247916_at

Mutant Information Allele Symbol bio 1-2 bio 1-3 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Syngenta Mutant Line Number 36172 46455 Ecotype Columbia Columbia Genetic Background     ABRC Stock CS16001 CS16002 Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence Details  (Help) Details Location of Mutation Exon 8 Downstream to At5g57580 Terminal Phenotype Cotyledon  Details Cotyledon  Details Nomarski Phenotype   Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release March, 2002 March, 2002 Seed Color Pale Yellow White Embryo Color Pale Yellow White Percent Mutant Seeds 27.0 % 34.8 % Chi - Square 3.3 51.0 Seeds Counted 1045 994 Percent Top Half 48.9 % 55.8 % Chi - Square 0.1 2.3 Average Seed Length 510 ± 40 µm 510 ± 40 µm Average Embryo Length 300 ± 100 µm 320 ± 90 µm