SeedGenes Profile: CH 42

Gene Information Gene Symbol CH 42 Chromosome Locus At4g18480   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol PDE 314 (D. Meinke) Alternate Name Gene Class Seed Pigment Predicted Function Magnesium Chelatase (CHLI) Function Details First committed step in chlorophyll biosynthesis; Insertion of magnesium into protoporphyrin IX Gene Evidence Sequenced Mutant Allele B. Pogson (1 allele) Double Border Recovery Meinke / Syngenta (1 allele) Database Release September, 2002 Predicted Sequences Gene: 1798 nucleotides mRNA: 1608 nucleotides Protein:   424 amino acids cDNA Status FLcDNA Available: AY093192 Top BLAST Hits Arabidopsis: AT5G45930.1 (83%) Plant ESTs: Brassica napus (94%) Model Genomes:   Synechocystis (73%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 254623_at

Mutant Information Allele Symbol ch 42-1 ch 42-1S Mutant Class Seed Pigment Seed Pigment Source of Mutant J. Relichova Meinke / Syngenta Mutant Line Number   99807 Ecotype Uncertain Columbia Genetic Background     ABRC Stock CS44 CS16119 Mutagen Treatment Irradiation T-DNA Insertion Mutant No Yes   Details Flanking Sequence   Details  (Help) Location of Mutation Exon 3 Intron 2 Terminal Phenotype Pigment Defective Pigment Defective Nomarski Phenotype Allelism Evidence Reference Allele Sequence Identity Database Release September, 2002 September, 2002 Seed Color Pale Yellow Pale Yellow Embryo Color Pale Yellow Pale Yellow Percent Mutant Seeds 26.3 % 23.8 % Chi - Square 0.9 0.7 Seeds Counted 923 952 Percent Top Half 49.8 % 49.8 % Chi - Square <0.1 <0.1 Average Seed Length     Average Embryo Length