OSU Syngenta vbi

SeedGenes Profile: EMB 1067

Gene Information
Gene Symbol EMB 1067
Chromosome Locus At2g45330   tair
Alleles in Database 2   See List OR Show All
Identity Confidence Confirmed
Alias Symbol TRPT 1 (D. Meinke) Alternate Name
Gene Class Embryo Defective
Predicted Function 2' tRNA Phosphotransferase
Function Details Catalytic role in tRNA splicing
Gene Evidence
Double Border Recovery
Meinke / Syngenta (1 allele)
Border Recovery
Meinke / Riken (1 allele)
Database Release March, 2002
Predicted Sequences
Gene:1700 nucleotides
mRNA: 1035 nucleotides
Protein:  289 amino acids
cDNA Status FLcDNA Available: AK228262
Top BLAST Hits
Arabidopsis: AT5G23600.1 (95%)
Plant ESTs: Brassica napus (93%)
Model Genomes:  Danio rerio (41%)
Subcellular Localization TargetP Targeted to Mitochondria
SUBA
mRNA Expression Data NASCArray Spot History for 245131_s_at
    
Mutant Information
Allele Symbol emb 1067-1emb 1067-2
Mutant Class Embryo DefectiveEmbryo Defective
Source of Mutant Meinke / SyngentaMeinke / Riken
Mutant Line Number 7724RATM13-1397-1
Ecotype ColumbiaNossen
Genetic Background   
ABRC Stock CS16010 
Mutagen Treatment T-DNATransposon
Insertion Mutant Yes   DetailsYes   Details
Flanking Sequence Details  (Help) 
Location of Mutation Exon 6 
Terminal Phenotype Cotyledon  DetailsGlobular
Nomarski Phenotype
Allelism Evidence Reference AlleleComplementation Test; Sequence Identity
Database Release March, 2002December, 2010
Seed Color Pale Yellow-Green 
Embryo Color Pale Yellow-Green 
Percent Mutant Seeds 23.4 %26.0 %
Chi - Square 1.30.2
Seeds Counted 1016570
Percent Top Half 50.4 % 
Chi - Square <0.1 
Average Seed Length 490 ± 60 µm 
Average Embryo Length 290 ± 80 µm