SeedGenes Profile: EMB 1427

Gene Information Gene Symbol EMB 1427 Chromosome Locus At1g80260   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Gamma Tubulin (GCP5) Function Details Microtubule nucleation Gene Evidence Double Border Recovery Meinke / Syngenta (2 alleles) Database Release March, 2002 Predicted Sequences Gene: 4897 nucleotides mRNA: 3297 nucleotides Protein:   995 amino acids cDNA Status FLcDNA Available: AK228920 Top BLAST Hits Arabidopsis: AT1G20570.1 (81%) Plant ESTs: Rice (Oryza sativa) (48%) Model Genomes:   None Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 262062_s_at

Mutant Information Allele Symbol emb 1427-1 emb 1427-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Syngenta Mutant Line Number 21991 65508 Ecotype Columbia Columbia Genetic Background   qrt ABRC Stock CS16019 CS16144 Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence Details  (Help) Details Location of Mutation Exon 6 Exon 4 Terminal Phenotype Cotyledon  Details Cotyledon  Details Nomarski Phenotype   Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release March, 2002 September, 2002 Seed Color White to Pale Yellow Pale Yellow-Green Embryo Color White to Pale Yellow Pale Yellow-Green Percent Mutant Seeds 17.7 % 20.8 % Chi - Square 30.9 11.1 Seeds Counted 1076 1156 Percent Top Half 33.7 % 52.5 % Chi - Square 20.2 0.6 Average Seed Length 520 ± 60 µm 560 ± 130 µm Average Embryo Length 140 ± 140 µm 210 ± 150 µm