SeedGenes Profile: EMB 1873

Gene Information Gene Symbol EMB 1873 Chromosome Locus At5g08170   Alleles in Database 3   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Agmatine Iminohydrolase Function Details Polyamine biosynthesis Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Single Border Recovery Meinke / Salk (2 alleles) Database Release September, 2002 Predicted Sequences Gene: 2771 nucleotides mRNA: 1538 nucleotides Protein:   383 amino acids cDNA Status FLcDNA Available: AK118589 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (89%) Model Genomes:   None Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 250570_at

Mutant Information Allele Symbol emb 1873-1 emb 1873-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Salk Mutant Line Number 43301 SALK_138993 Ecotype Columbia Columbia Genetic Background     ABRC Stock CS16128 CS24077 Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence Details  (Help)   Location of Mutation Intron 1 Exon 4 Terminal Phenotype Cotyledon  Details Cotyledon  Details Nomarski Phenotype   Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release September, 2002 March, 2004 Seed Color Pale Yellow Pale Green Embryo Color Pale Yellow Pale Green Percent Mutant Seeds 26.6 % 25.7 % Chi - Square 1.4 0.3 Seeds Counted 1011 1137 Percent Top Half 52.4 % 58.9 % Chi - Square 0.6 9.2 Average Seed Length 470 ± 50 µm 500 ± 50 µm Average Embryo Length 260 ± 80 µm 370 ± 50 µm