SeedGenes Profile: EMB 2296

Gene Information Gene Symbol EMB 2296 Chromosome Locus At2g18020   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Ribosomal Protein L8/L2 Function Details Translation in mitochondria or cytoplasm Gene Evidence Double Border Recovery Meinke / Syngenta (2 alleles) Database Release March, 2002 Predicted Sequences Gene: 1202 nucleotides mRNA: 969 nucleotides Protein:   258 amino acids cDNA Status FLcDNA Available: BX820387 Top BLAST Hits Arabidopsis: AT4G36130.1 (96%) Plant ESTs: Brassica napus (96%) Model Genomes:   Drosophila melanogaster (72%) Subcellular Localization TargetP Targeted to Mitochondria SUBA mRNA Expression Data NASCArray Spot History for 265805_s_at

Mutant Information Allele Symbol emb 2296-1 emb 2296-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Syngenta Mutant Line Number 78861 86141 Ecotype Columbia Columbia Genetic Background qrt   ABRC Stock CS16050 CS16051 Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence Details  (Help) Details Location of Mutation Exon 2 Exon 2 to 3' UTR Terminal Phenotype Globular  Details Globular  Details Nomarski Phenotype   Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release March, 2002 March, 2002 Seed Color White White Embryo Color White White Percent Mutant Seeds 30.0 % 21.1 % Chi - Square 13.1 7.7 Seeds Counted 991 953 Percent Top Half 54.5 % 55.7 % Chi - Square 2.4 2.6 Average Seed Length 450 ± 40 µm 440 ± 30 µm Average Embryo Length 60 ± 30 µm 110 ± 50 µm