OSU Syngenta vbi

SeedGenes Profile: EMB 2407

Gene Information
Gene Symbol EMB 2407
Chromosome Locus At5g60540   tair
Alleles in Database 3   See List OR Show All
Identity Confidence Confirmed
Alias Symbol PDX 2 (T. Fitzpatrick) Sequence Identity
Gene Class Embryo Defective
Predicted Function Imidazoleglycerol-Phosphate Synthase Subunit H; Pyroxidine Amidotransferase
Function Details Pyroxidine (vitamin B6) biosynthesis
Gene Evidence
Double Border Recovery
Meinke / Syngenta (1 allele)
Border Recovery
Fitzpatrick / Salk (1 allele)
Border Recovery
Fitzpatrick / Riken (1 allele)
Database Release September, 2003
Predicted Sequences
Gene:2180 nucleotides
mRNA: 1174 nucleotides
Protein:  255 amino acids
cDNA Status FLcDNA Available: AK117313
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Brassica napus (91%)
Model Genomes:  Neurospora crassa (44%)
Subcellular Localization TargetP Targeted to Mitochondria
SUBA
mRNA Expression Data NASCArray Spot History for 247641_at
    
Mutant Information
Allele Symbol emb 2407pdx 2-1
Mutant Class Embryo DefectiveEmbryo Defective
Source of Mutant Meinke / SyngentaFitzpatrick / Salk
Mutant Line Number 85146SALK_072168
Ecotype ColumbiaColumbia
Genetic Background   
ABRC Stock CS16214 
Mutagen Treatment T-DNAT-DNA
Insertion Mutant Yes   DetailsYes   Details
Flanking Sequence Details  (Help) 
Location of Mutation Exon 2Intron 1
Terminal Phenotype Cotyledon  DetailsGlobular
Nomarski Phenotype   Images
Allelism Evidence Reference AlleleSequence Identity
Database Release September, 2003October, 2007
Seed Color White to Pale Yellow 
Embryo Color White to Pale Yellow 
Percent Mutant Seeds 26.2 % 
Chi - Square 0.8 
Seeds Counted 1086 
Percent Top Half 51.1 % 
Chi - Square 0.1 
Average Seed Length 490 ± 70 µm 
Average Embryo Length 160 ± 90 µm